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anti-Human IL7R antibody for Immunohistochemistry (Frozen Sections)

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • CD127
  • CDW127
  • IL-7R-alpha
  • IL-7RA
  • IL-7Ralpha
  • IL7R
  • IL7RA
  • ILRA
Epitope
AA 173-260
24
13
13
10
6
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
164
145
27
3
Host
Rabbit
93
93
67
12
2
Clonality
Polyclonal
Conjugate
This IL7R antibody is un-conjugated
25
20
17
16
10
10
9
9
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
118
89
63
24
22
13
12
11
11
8
5
4
4
3
3
3
3
2
2
1
1
Supplier
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Catalog No. ABIN1859442
$ 365.20
Plus shipping costs $45.00

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Application / Reactivity Human
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ELISA 36 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 50 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Multiplex Assay (MA) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Stimulation (St) 2 Antibodies
Western Blotting (WB) 65 Antibodies

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Epitope AA 173-260
(24), (13), (13), (10), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(164), (145), (27), (3)
Host Rabbit
(93), (93), (67), (12), (2)
Clonality
Polyclonal
Conjugate This IL7R antibody is un-conjugated
(25), (20), (17), (16), (10), (10), (9), (9), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(118), (89), (63), (24), (22), (13), (12), (11), (11), (8), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1)
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Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-KDENKWTH VNLSSTKLTL LQRKLQPAAM YEIKVRSIPD HYFKGFWSEW SPSYYFRTPE INNSSGEMDP ILLTISILSF FSVALLVILA
Specificity It has been selected for its ability to recognize IL7R in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL7R (AA 173-260)
Isotype IgG

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL7R (Lys173~Ala260) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL7R antibody (Interleukin 7 Receptor) (AA 173-260) (ABIN1859442) anti-Interleukin 7 Receptor (IL7R) (AA 173-260) antibody