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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Reagent (BR)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||4 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||69 Antibodies|
|Functional Studies (Func)||3 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Intracellular Flow Cytometry (ICFC)||12 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Western Blotting (WB)||51 Antibodies|
|Antigen||Interleukin 7 Receptor (IL7R) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL7R antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-IL7R AntibodyTarget Details IL7R Application Details Handling References for anti-IL7R Antibody (ABIN1176855) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||BALB/c mouse pre-B cell line 1A9|
Target Details IL7RProduct Details anti-IL7R Antibody Application Details Handling References for anti-IL7R Antibody (ABIN1176855) Images back to top
|Alternative Name||CD127 (IL7R Antibody Abstract)|
The SB/14 monoclonal antibody reacts with CD127, the 65-75-kDa type-I transmembrane protein IL-7Ralpha. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Ralpha and CD132, the common gamma chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Ralpha is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density gammadelta TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring alphabeta TCR-bearing T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation). Blocking of IL-7-induced lymphocyte proliferation. 2E8 cells (mouse immature B lymphocyte cell line) were cultured for 48 hours in the presence of recombinant mouse IL-7 (blue histogram), with mAb SB/14 added 30 minutes prior to IL-7 (red histogram), or in the absence of IL-7 (green histogram). Proliferation was detected by incorporation of bromodeoxyuridine (BrdU) using the BrdU Flow Kit, Cat. No. 559619. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Synonyms: IL-7 Receptor alpha chain
Application DetailsProduct Details anti-IL7R Antibody Target Details IL7R Handling References for anti-IL7R Antibody (ABIN1176855) Images back to top
|Application Notes||Flow cytometry: Since this antigen is expressed at low density on lymphoid cell surfaces, it may be desirable to amplify staining by using a bright second-step reagent. If a three-step method is employed, the use of anti-Mouse CD16/CD32, Mouse Fc Block™ (Cat. No. 553141) is recommended to reduce non-specific staining. Special precaution should be taken to utilize a second-step antibody which does not crossreact with 2.4G2 mAb (rat IgG2b, kappa), such as biotinylated anti-rat IgG2a (RG7/1.30, Cat. No. 553894) followed by PE Streptavidin (Cat. No. 554061).|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL7R Antibody Target Details IL7R Application Details References for anti-IL7R Antibody (ABIN1176855) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-IL7R Antibody (ABIN1176855)Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling Images back to top
|Product cited in:||
Borghesi, Yamashita, Kincade: "Heparan sulfate proteoglycans mediate interleukin-7-dependent B lymphopoiesis." in: Blood, Vol. 93, Issue 1, pp. 140-8, 1999 (PubMed).
Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, 1999 (PubMed).
Fujihashi, Kawabata, Hiroi et al.: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, 1996 (PubMed).
Sudo, Nishikawa, Ohno et al.: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, 1993 (PubMed).
Noguchi, Nakamura, Russell et al.: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, 1994 (PubMed).
Peschon, Morrissey, Grabstein et al.: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, 1994 (PubMed).
Faust, Saffran, Toksoz et al.: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, 1993 (PubMed).
Goodwin, Friend, Ziegler et al.: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, 1990 (PubMed).
Henderson, Narayanan, Collins et al.: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, 1992 (PubMed).
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