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anti-Mouse (Murine) IL7R antibody for Flow Cytometry

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • CD127
  • CDW127
  • IL-7R-alpha
  • IL-7RA
  • IL-7Ralpha
  • IL7R
  • IL7RA
  • ILRA
Reactivity
Mouse (Murine)
163
146
27
3
Host
Rat
94
92
67
12
2
Clonality
Monoclonal
Conjugate
This IL7R antibody is un-conjugated
25
20
17
16
10
10
9
9
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
117
89
64
23
22
13
12
12
10
9
4
4
4
4
3
3
3
2
2
1
1
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Catalog No. ABIN1176855
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29422182 ABIN2658912 FACS PE-Cy5 Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN2659647 FACS PE/Dazzle™ 594 Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN2660810 BR FACS IP IHC Biotin Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN2660936 FACS Biotin Rat IgG2b kappa Log in to see SB-199 9
0.29422182 ABIN2658470 FACS APC Rat IgG2b kappa Log in to see SB-199 9
0.29422182 ABIN2658467 FACS APC Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN2657324 FACS Alexa Fluor 488 Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN2657326 FACS ICFC Alexa Fluor 488 Rat IgG2b kappa Log in to see SB-199 9
0.29422182 ABIN2664192 FACS CyTOF Rat IgG2a kappa Log in to see A7R34 5
0.29422182 ABIN1032245 FACS APC eFluor 780 Rat IgG2a kappa Log in to see A7R34
0.29422182 ABIN1032246 FACS EFluor 450 Rat IgG2a kappa Log in to see A7R34
0.29422182 ABIN2677447 FACS APC Rat IgG2b kappa Log in to see SB-199 (SB-199)
0.29422182 ABIN2677455 FACS EFluor 660 Rat IgG2a kappa Log in to see A7R34
0.29422182 ABIN2677449 FACS PE-Cy7 Rat IgG2b kappa Log in to see SB-199 (SB-199)
0.29422182 ABIN2677451 FACS PerCP-eFluor® 710 Rat IgG2b kappa Log in to see SB-199 (SB-199)
0.29422182 ABIN2677453 FACS EFluor 450 Rat IgG2b kappa Log in to see SB-199 (SB-199)
0.0010489733 ABIN286591 FACS Alexa Fluor 488 Rat IgG2a kappa Log in to see A7R34 1
0.0010489733 ABIN2688949 FACS Biotin Rat IgG2a, lambda Log in to see B12 8
0.0010489733 ABIN1832292 FACS APC Goat IgG Extracellular Domain Log in to see Polyclonal
0.0010489733 ABIN282324 Func FACS Rat IgG2a kappa Log in to see A7R34

Top referenced anti-IL7R antibody for Flow Cytometry

Similar anti-IL7R Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 37 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 4 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 69 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 12 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 51 Antibodies

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Reactivity Mouse (Murine)
(163), (146), (27), (3)
Host Rat
(94), (92), (67), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL7R antibody is un-conjugated
(25), (20), (17), (16), (10), (10), (9), (9), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(117), (89), (64), (23), (22), (13), (12), (12), (10), (9), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 9 references available
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Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling References for anti-IL7R Antibody (ABIN1176855) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen BALB/c mouse pre-B cell line 1A9
Clone SB-14
Isotype IgG2a, kappa

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling References for anti-IL7R Antibody (ABIN1176855) Images back to top
Antigen
Alternative Name CD127 (IL7R Antibody Abstract)
Background The SB/14 monoclonal antibody reacts with CD127, the 65-75-kDa type-I transmembrane protein IL-7Ralpha. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Ralpha and CD132, the common gamma chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Ralpha is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density gammadelta TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring alphabeta TCR-bearing T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation). Blocking of IL-7-induced lymphocyte proliferation. 2E8 cells (mouse immature B lymphocyte cell line) were cultured for 48 hours in the presence of recombinant mouse IL-7 (blue histogram), with mAb SB/14 added 30 minutes prior to IL-7 (red histogram), or in the absence of IL-7 (green histogram). Proliferation was detected by incorporation of bromodeoxyuridine (BrdU) using the BrdU Flow Kit, Cat. No. 559619. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Synonyms: IL-7 Receptor alpha chain
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling References for anti-IL7R Antibody (ABIN1176855) Images back to top
Application Notes Flow cytometry: Since this antigen is expressed at low density on lymphoid cell surfaces, it may be desirable to amplify staining by using a bright second-step reagent. If a three-step method is employed, the use of anti-Mouse CD16/CD32, Mouse Fc Block™ (Cat. No. 553141) is recommended to reduce non-specific staining. Special precaution should be taken to utilize a second-step antibody which does not crossreact with 2.4G2 mAb (rat IgG2b, kappa), such as biotinylated anti-rat IgG2a (RG7/1.30, Cat. No. 553894) followed by PE Streptavidin (Cat. No. 554061).
Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details References for anti-IL7R Antibody (ABIN1176855) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL7R Antibody (ABIN1176855)

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling Images back to top
Product cited in:

Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, 1999

Borghesi, Yamashita, Kincade: "Heparan sulfate proteoglycans mediate interleukin-7-dependent B lymphopoiesis." in: Blood, Vol. 93, Issue 1, pp. 140-8, 1999

Fujihashi, Kawabata, Hiroi, Yamamoto, McGhee, Nishikawa, Kiyono: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, 1996

Noguchi, Nakamura, Russell, Ziegler, Tsang, Cao, Leonard: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, 1994

Peschon, Morrissey, Grabstein, Ramsdell, Maraskovsky, Gliniak, Park, Ziegler, Williams, Ware, Meyer, Davison: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, 1994

Faust, Saffran, Toksoz, Williams, Witte: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, 1993

Sudo, Nishikawa, Ohno, Akiyama, Tamakoshi, Yoshida, Nishikawa: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, 1993

Henderson, Narayanan, Collins, Dorshkind: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, 1992

Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, 1990

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling References for anti-IL7R Antibody (ABIN1176855) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL7R antibody (Interleukin 7 Receptor) (ABIN1176855) anti-Interleukin 7 Receptor (IL7R) antibody