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anti-Mouse (Murine) LIF antibody for Immunocytochemistry

Recommended LIF Antibody (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) Antibodies
  • A230075M04Rik
  • AW061234
  • CDF
  • DIA
  • HILDA
  • LIF
  • MLPLI
Epitope
AA 25-203
15
13
8
6
5
5
4
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
103
60
23
6
6
4
4
Host
Rabbit
88
20
15
3
2
Clonality
Polyclonal
Conjugate
This LIF antibody is un-conjugated
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
107
39
31
25
22
3
3
3
3
3
2
1
1
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Catalog No. ABIN1859654
$ 356.40
Plus shipping costs $45.00

Similar anti-LIF Antibodies

Application / Reactivity Mouse (Murine)
ELISA 16 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 52 Antibodies

General

Antigen Leukemia Inhibitory Factor (LIF) Antibodies
Epitope AA 25-203
(15), (13), (8), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(103), (60), (23), (6), (6), (4), (4)
Host Rabbit
(88), (20), (15), (3), (2)
Clonality
Polyclonal
Conjugate This LIF antibody is un-conjugated
(4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(107), (39), (31), (25), (22), (3), (3), (3), (3), (3), (2), (1), (1)
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Product Details anti-LIF Antibody

Target Details LIF Application Details Handling Images
Sequence MGHHHHHHSG SEF- PLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF
Specificity It has been selected for its ability to recognize LIF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen LIF (AA 25-203)
Isotype IgG

Target Details LIF

Product Details anti-LIF Antibody Application Details Handling Images back to top
Antigen
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-LIF Antibody Target Details LIF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant LIF (Pro25~Phe203) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-LIF Antibody Target Details LIF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-LIF Antibody Target Details LIF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-LIF antibody (Leukemia Inhibitory Factor) (AA 25-203) (ABIN1859654) anti-Leukemia Inhibitory Factor (LIF) (AA 25-203) antibody