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anti-Human MPL antibody for ELISA

Recommended MPL Antibody (supplied by: Log in to see )

Antigen
Myeloproliferative Leukemia Virus Oncogene (MPL) Antibodies
  • C-MPL
  • c-mpl
  • c-mpl-I
  • c-mpl-II
  • CD110
  • hlb219
  • MPL
  • MPLV
  • THCYT2
  • TPO-R
  • TPOR
Reactivity
Human
51
33
28
Host
Mouse
45
10
Clonality
Monoclonal
Conjugate
This MPL antibody is un-conjugated
4
4
3
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
27
22
11
10
8
3
2
2
2
1
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Catalog No. ABIN969542
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30628014 ABIN1844476 FACS ELISA WB Mouse IgG2b Log in to see
0.0012155735 ABIN1497161 FACS ELISA WB Mouse IgG2b Log in to see 1H2
0.0012155735 ABIN1859873 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 311-460 Log in to see Polyclonal
0.0012155735 ABIN3187963 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012155735 ABIN3183729 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012155735 ABIN2618330 ELISA WB Biotin Rabbit IgG AA 311-460 Log in to see Polyclonal
0.0012155735 ABIN2592667 ELISA Rabbit IgG AA 311-460 Log in to see Polyclonal
0.0012155735 ABIN2325642 FACS ELISA WB Mouse IgG2b Log in to see 13B530

Top referenced anti-MPL antibody for ELISA

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Application / Reactivity Human
ELISA 9 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 25 Antibodies

General

Antigen Myeloproliferative Leukemia Virus Oncogene (MPL) Antibodies
Reactivity Human
(51), (33), (28)
Host Mouse
(45), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MPL antibody is un-conjugated
(4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(27), (22), (11), (10), (8), (3), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-MPL Antibody

Target Details MPL Application Details Handling References for anti-MPL Antibody (ABIN969542) Images
Purification Purified
Immunogen Purified recombinant fragment of human MPL expressed in E. coli.
Clone 1H2
Isotype IgG2b

Target Details MPL

Product Details anti-MPL Antibody Application Details Handling References for anti-MPL Antibody (ABIN969542) Images back to top
Antigen
Alternative Name MPL (MPL Antibody Abstract)
Background In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Molecular Weight 71.2 kDa
Gene ID 4352
HGNC 4352
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-MPL Antibody Target Details MPL Handling References for anti-MPL Antibody (ABIN969542) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-MPL Antibody Target Details MPL Application Details References for anti-MPL Antibody (ABIN969542) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-MPL Antibody (ABIN969542)

Product Details anti-MPL Antibody Target Details MPL Application Details Handling Images back to top
Product cited in:

Marty, Chaligné, Lacout, Constantinescu, Vainchenker, Villeval: "Ligand-independent thrombopoietin mutant receptor requires cell surface localization for endogenous activity." in: The Journal of biological chemistry, Vol. 284, Issue 18, pp. 11781-91, 2009

Blackford, Parmigiani, Kensler, Wolfgang, Jones, Zhang, Parsons, Lin, Leary, Eshleman, Goggins, Jaffee, Iacobuzio-Donahue, Maitra, Klein, Cameron, Olino, Schulick, Winter, Vogelstein, Velculescu, Kinzler, Hruban: "Genetic mutations associated with cigarette smoking in pancreatic cancer." in: Cancer research, Vol. 69, Issue 8, pp. 3681-8, 2009

Images

Product Details anti-MPL Antibody Target Details MPL Application Details Handling References for anti-MPL Antibody (ABIN969542) back to top
Supplier Images
ELISA image for anti-MPL antibody (Myeloproliferative Leukemia Virus Oncogene) (ABIN969542) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-MPL antibody (Myeloproliferative Leukemia Virus Oncogene) (ABIN969542) Figure 2: Flow cytometric analysis of MOLT4 cells using MPL mouse mAb (green) and neg...
Western Blotting (WB) image for anti-MPL antibody (Myeloproliferative Leukemia Virus Oncogene) (ABIN969542) Figure 3: Western blot analysis using MPL mAb against human MPL (AA: 307-362) recombi...