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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||25 Antibodies|
|Antigen||Myeloproliferative Leukemia Virus Oncogene (MPL) Antibodies|
|Conjugate||This MPL antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-MPL AntibodyTarget Details MPL Application Details Handling References for anti-MPL Antibody (ABIN969542) Images
|Immunogen||Purified recombinant fragment of human MPL expressed in E. coli.|
Target Details MPLProduct Details anti-MPL Antibody Application Details Handling References for anti-MPL Antibody (ABIN969542) Images back to top
|Alternative Name||MPL (MPL Antibody Abstract)|
|Background||In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.|
|Molecular Weight||71.2 kDa|
Application DetailsProduct Details anti-MPL Antibody Target Details MPL Handling References for anti-MPL Antibody (ABIN969542) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MPL Antibody Target Details MPL Application Details References for anti-MPL Antibody (ABIN969542) Images back to top
|Buffer||PBS with 0.05 % sodium azide and 0.5 % protein stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-MPL Antibody (ABIN969542)Product Details anti-MPL Antibody Target Details MPL Application Details Handling Images back to top
|Product cited in:||
Blackford, Parmigiani, Kensler et al.: "Genetic mutations associated with cigarette smoking in pancreatic cancer." in: Cancer research, Vol. 69, Issue 8, pp. 3681-8, 2009 (PubMed).
Marty, Chaligné, Lacout et al.: "Ligand-independent thrombopoietin mutant receptor requires cell surface localization for endogenous activity." in: The Journal of biological chemistry, Vol. 284, Issue 18, pp. 11781-91, 2009 (PubMed).
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