anti-Human MPL antibody for Flow Cytometry

Recommended MPL Antibody (supplied by: Log in to see )

Antigen
Myeloproliferative Leukemia Virus Oncogene (MPL) Antibodies
  • C-MPL
  • CD110
  • MPLV
  • THCYT2
  • TPOR
  • TPO-R
  • c-mpl
  • c-mpl-I
  • c-mpl-II
  • hlb219
  • MPL
  • mpll
  • tpor
  • tpor.b
  • MPL proto-oncogene, thrombopoietin receptor
  • myeloproliferative leukemia virus oncogene
  • MPL
  • Mpl
  • mpl
Reactivity
Human
61
39
32
1
1
Host
Mouse
51
15
Clonality
Monoclonal
Conjugate
This MPL antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
33
29
26
13
7
6
5
2
1
Options
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Catalog No. ABIN969542
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.098416 ABIN1105749 EIA FACS WB Mouse IgG2b Log in to see 1H2 2
14.098416 ABIN4896877 FACS PE Mouse IgG2a AA 25-423 Log in to see 167639
14.098416 ABIN4896876 FACS APC Mouse IgG2a AA 25-423 Log in to see 167639
14.098416 ABIN1387832 FACS IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
14.098416 ABIN517994 FACS WB Mouse AA 1-635, full length Log in to see Polyclonal
14.098416 ABIN1844476 FACS ELISA WB Mouse IgG2b Log in to see
1 ABIN967682 FACS WB Mouse IgG1, kappa Extracellular Domain Log in to see 1-78-1 4
1 ABIN1497161 FACS ELISA WB Mouse IgG2b Log in to see 1H2
1 ABIN4880878 ELISA FACS WB Mouse IgG2b Log in to see 1H2
1 ABIN797279 FACS PE Mouse IgG2a Log in to see
1 ABIN1425855 FACS PE Rabbit IgG Log in to see Polyclonal
1 ABIN1408408 FACS IF (p) Cy5 Rabbit IgG Log in to see Polyclonal
1 ABIN1408407 FACS IF (p) Cy3 Rabbit IgG Log in to see Polyclonal
1 ABIN1408409 FACS IF (p) Cy5.5 Rabbit IgG Log in to see Polyclonal
1 ABIN1390516 FACS IF (p) Alexa Fluor 488 Rabbit IgG Log in to see Polyclonal
1 ABIN1390515 FACS IF (p) Alexa Fluor 350 Rabbit IgG Log in to see Polyclonal
1 ABIN1390518 FACS IF (p) Alexa Fluor 647 Rabbit IgG Log in to see Polyclonal
1 ABIN1390517 FACS IF (p) Alexa Fluor 555 Rabbit IgG Log in to see Polyclonal
1 ABIN1390520 FACS IF (p) FITC Rabbit IgG Log in to see Polyclonal
1 ABIN1408410 FACS IF (p) Cy7 Rabbit IgG Log in to see Polyclonal

General

Antigen Myeloproliferative Leukemia Virus Oncogene (MPL) Antibodies
Reactivity Human
(61), (39), (32), (1), (1)
Host Mouse
(51), (15)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MPL antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(33), (29), (26), (13), (7), (6), (5), (2), (1)
Pubmed 2 references available
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Product Details anti-MPL Antibody

Target Details MPL Application Details Handling References for anti-MPL antibody (ABIN969542) Images
Purification Purified
Immunogen Purified recombinant fragment of human MPL expressed in E. Coli.
Clone 1H2
Isotype IgG2b

Target Details MPL

Product Details anti-MPL Antibody Application Details Handling References for anti-MPL antibody (ABIN969542) Images back to top
Antigen
Alternative Name MPL (MPL Antibody Abstract)
Background In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Molecular Weight 71.2 kDa
Gene ID 4352
HGNC 4352
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-MPL Antibody Target Details MPL Handling References for anti-MPL antibody (ABIN969542) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-MPL Antibody Target Details MPL Application Details References for anti-MPL antibody (ABIN969542) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-MPL antibody (ABIN969542)

Product Details anti-MPL Antibody Target Details MPL Application Details Handling Images back to top
Product cited in:

Marty, Chaligné, Lacout, Constantinescu, Vainchenker, Villeval: "Ligand-independent thrombopoietin mutant receptor requires cell surface localization for endogenous activity." in: The Journal of biological chemistry, Vol. 284, Issue 18, pp. 11781-91, 2009

Blackford, Parmigiani, Kensler, Wolfgang, Jones, Zhang, Parsons, Lin, Leary, Eshleman, Goggins, Jaffee, Iacobuzio-Donahue, Maitra, Klein, Cameron, Olino, Schulick, Winter, Vogelstein, Velculescu, Kinzler, Hruban: "Genetic mutations associated with cigarette smoking in pancreatic cancer." in: Cancer research, Vol. 69, Issue 8, pp. 3681-8, 2009

Images

Product Details anti-MPL Antibody Target Details MPL Application Details Handling References for anti-MPL antibody (ABIN969542) back to top
Supplier Images
ELISA image for anti-Myeloproliferative Leukemia Virus Oncogene (MPL) antibody (ABIN969542) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Myeloproliferative Leukemia Virus Oncogene (MPL) antibody (ABIN969542) Figure 2: Flow cytometric analysis of MOLT4 cells using MPL mouse mAb (green) and neg...
Western Blotting (WB) image for anti-Myeloproliferative Leukemia Virus Oncogene (MPL) antibody (ABIN969542) Figure 3: Western blot analysis using MPL mAb against human MPL (AA: 307-362) recombi...