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anti-Rabbit Prolactin Receptor antibody for Immunohistochemistry (Frozen Sections)

Recommended Prolactin Receptor Antibody (supplied by: Log in to see )

Antigen
Prolactin Receptor (PRLR) Antibodies
  • AI987712
  • DKFZp469D061
  • hPRLrI
  • Pr-1
  • Pr-3
  • PRL-R
  • PRLR
  • prlr
  • Prlr-rs1
  • PRLRb
  • RATPRLR
  • tprlr
Reactivity
Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
200
59
30
14
13
13
13
13
13
Host
Mouse
163
57
7
4
Clonality
Monoclonal
Conjugate
This Prolactin Receptor antibody is un-conjugated
7
7
7
7
6
6
6
6
6
6
6
6
6
6
5
4
4
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
176
121
115
101
97
90
81
57
35
11
2
2
2
1
1
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Catalog No. ABIN152720
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088411616 ABIN4347618 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347620 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347621 FACS ICC IF IHC IHC (fro) IHC (p) IP WB PE Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347622 FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347624 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347626 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347628 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347630 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405LS Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347632 FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347634 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347636 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347638 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 Log in to see U5
0.00088411616 ABIN4347640 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 Log in to see U5

Similar anti-Prolactin Receptor Antibodies

Application / Reactivity Rat (Rattus) Rabbit
ELISA 9 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies 14 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 31 Antibodies 14 Antibodies
Immunofluorescence (IF) 29 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 17 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies 14 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 46 Antibodies

General

Antigen Prolactin Receptor (PRLR) Antibodies
Reactivity Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
(200), (59), (30), (14), (13), (13), (13), (13), (13)
Host Mouse
(163), (57), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Prolactin Receptor antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(176), (121), (115), (101), (97), (90), (81), (57), (35), (11), (2), (2), (2), (1), (1)
Pubmed 10 references available
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Product Details anti-Prolactin Receptor Antibody

Target Details Prolactin Receptor Application Details Handling References for anti-Prolactin Receptor Antibody (ABIN152720) Images
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen Purified rat liver PRL receptor.
Clone U5
Isotype IgG1

Target Details Prolactin Receptor

Product Details anti-Prolactin Receptor Antibody Application Details Handling References for anti-Prolactin Receptor Antibody (ABIN152720) Images back to top
Antigen
Alternative Name Prolactin R (PRLR Antibody Abstract)
Background Prolactin (PRL) is a hormone involved in a variety of important functions including iontransport and osmoregulation, stimulation of milk, protein synthesis as well as theregulation of numerous reproductive functions. PRL exerts its influence on different celltypes through a signal transduction pathway which begins with the binding of thehormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a memberof the cytokine receptor family, varies in size (short and long forms) with tissue sourceand species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least threeseparate domains: an extracellular region with 5 cysteines which contains the prolactinbinding site, a single transmembrane domain and a cytoplasmic region, the length ofwhich appears to influence ligand binding and regulate cellular function. Alternate Names: Anti-CLONE SPM213 antibody, anti-hPRL receptor antibody, anti-hPRLrI antibody, anti-PRL R antibody, anti- PRLR antibody.
Gene Symbol: PRLR
Gene ID 5618, 24684
Pathways JAK-STAT Signaling

Application Details

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling References for anti-Prolactin Receptor Antibody (ABIN152720) Images back to top
Application Notes This antibody can be used in Immunoprecipitation, Immunofluorescence, and Western Blot. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor. Recommended Starting Dilutions*: ICC: 5 µg/ml WB: 1 µg/ml *Optimal dilutions should be determined by the end user.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 5 µg/mL, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details References for anti-Prolactin Receptor Antibody (ABIN152720) Images back to top
Format Lyophilized
Reconstitution Reconstitute in 0.1 mL PBS.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

References for anti-Prolactin Receptor Antibody (ABIN152720)

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling Images back to top
Product cited in:

Perrot-Applanat, Gualillo, Buteau, Edery, Kelly: "Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation." in: Journal of cell science, Vol. 110 ( Pt 9), pp. 1123-32, 1997

Royster, Driscoll, Kelly, Freemark: "The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation." in: Endocrinology, Vol. 136, Issue 9, pp. 3892-900, 1995

Sorenson, Stout: "Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis." in: Endocrinology, Vol. 136, Issue 9, pp. 4092-8, 1995

Rui, Lebrun, Kirken, Kelly, Farrar: "JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization." in: Endocrinology, Vol. 135, Issue 4, pp. 1299-306, 1994

Dardenne, de Moraes, Kelly, Gagnerault: "Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry." in: Endocrinology, Vol. 134, Issue 5, pp. 2108-14, 1994

Gagnerault, Touraine, Savino, Kelly, Dardenne: "Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5673-81, 1993

Rozakis-Adcock, Kelly: "Mutational analysis of the ligand-binding domain of the prolactin receptor." in: The Journal of biological chemistry, Vol. 266, Issue 25, pp. 16472-7, 1991

Dardenne, Kelly, Bach, Savino: "Identification and functional activity of prolactin receptors in thymic epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 21, pp. 9700-4, 1991

Okamura, Zachwieja, Raguet, Kelly: "Characterization and applications of monoclonal antibodies to the prolactin receptor." in: Endocrinology, Vol. 124, Issue 5, pp. 2499-508, 1989

Katoh, Raguet, Zachwieja, Djiane, Kelly: "Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies." in: Endocrinology, Vol. 120, Issue 2, pp. 739-49, 1987

Images

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling References for anti-Prolactin Receptor Antibody (ABIN152720) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Prolactin Receptor antibody (PRLR) (ABIN152720) Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerha...