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anti-Human PTPN6 antibody for Flow Cytometry

Recommended PTPN6 Antibody (supplied by: Log in to see )

Antigen
Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies
  • DRPLA
  • HCP
  • hcp
  • HCPH
  • Hcph
  • HPTP1C
  • me
  • motheaten
  • PTP-1C
  • Ptp1C
  • Ptph6
  • SH-PTP1
  • SHP
  • SHP-1
  • Shp-1
  • SHP-1L
  • Shp1
  • SHP1
Epitope
C-Term
33
22
13
9
8
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
178
77
73
8
2
2
1
1
1
Host
Rabbit
113
33
32
Clonality
Monoclonal
Conjugate
This PTPN6 antibody is un-conjugated
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
132
74
53
29
26
23
8
7
7
6
1
Supplier
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Available images

Catalog No. ABIN258467
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Similar anti-PTPN6 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 15 Antibodies 16 Antibodies 75 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 16 Antibodies 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 36 Antibodies 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 12 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies
Western Blotting (WB) 45 Antibodies 48 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies

General

Antigen Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies
Epitope C-Term
(33), (22), (13), (9), (8), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(178), (77), (73), (8), (2), (2), (1), (1), (1)
Host Rabbit
(113), (33), (32)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PTPN6 antibody is un-conjugated
(7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(132), (74), (53), (29), (26), (23), (8), (7), (7), (6), (1)
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Product Details anti-PTPN6 Antibody

Target Details PTPN6 Application Details Handling Images
Specificity This antibody is predicted to detect isoforms 2 and 3 based on sequence analysis.
Purification Protein A purified
Immunogen A synthetic peptide corresponding to residues in the C-term of human DHP-1 was used as immunogen.
Clone Y476
Isotype IgG

Target Details PTPN6

Product Details anti-PTPN6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PTPN6 (PTPN6 Antibody Abstract)
Background SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosinephosphatase (PTP) predominantly expressed in hematopoietic cells, and regulatesmultiple signaling pathways involved in hematopoietic cell growth, differentiation andactivation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosolto plasma membrane and bind to the tyrosine-phosphorylated receptors through theirSH2 domains, becoming activated in the process (2). SHP-1 has been involved in thenegative regulation of various growth promoting receptors including receptor tyrosinekinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine basedreceptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5). Alternate Names: anti-70 kda SHP 1L protein antibody,anti-kda SHP1L protein antibody, anti-P antibody,anti-PH antibody, anti-matopoietic cell phosphatase antibody, anti-matopoietic cellprotein-tyrosine phosphatase antibody, anti-TP 1C antibody, anti-TP1C antibody,anti-otein tyrosine phosphatase 1C antibody, anti-protein tyrosine phosphatase nonreceptor type 6 antibody, anti-protein tyrosine phosphatase SHP 1 antibody, anti-proteintyrosine phosphatase SHP1 antibody,PTP 1C antibody, anti-PTP1C antibody,anti-PTPN 6 antibody, anti-PTPN6 antibody, anti-SH PTP 1 antibody, anti-SH PTP1antibody, anti-SHP 1 antibody, anti-SHP 1L antibody, anti-SHP1L antibody, anti-SHPTP1 antibody, anti-SHPTP1 antibody, anti-Tyrosine protein phosphatase non receptor type6 antibody.
Gene Symbol: PTPN6
Gene ID 5777, 23957, 116689
Pathways JAK-STAT Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway

Application Details

Product Details anti-PTPN6 Antibody Target Details PTPN6 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 65kDa.
Recommended dilutions: Flow Cytometry 1:20, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000
Protocol Protocol specific for PTPN6 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PTPN6 antibody (Protein-tyrosine Phosphatase 1C) (C-Term) (ABIN258467) Western blot analysis of anti-SHP-1 (C-term) RabMAb (cat. #110-57570) 1:1,000 dilutio...
Immunohistochemistry (IHC) image for anti-PTPN6 antibody (Protein-tyrosine Phosphatase 1C) (C-Term) (ABIN258467) Immunohistochemical analysis of paraffin-embedded human lymph node using anti-SHP-1 (...