|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||15 Antibodies||16 Antibodies||75 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||16 Antibodies||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies||40 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies||12 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||45 Antibodies||48 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Antigen||Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PTPN6 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-PTPN6 AntibodyTarget Details PTPN6 Application Details Handling Images
|Specificity||This antibody is predicted to detect isoforms 2 and 3 based on sequence analysis.|
|Purification||Protein A purified|
|Immunogen||A synthetic peptide corresponding to residues in the C-term of human DHP-1 was used as immunogen.|
Target Details PTPN6Product Details anti-PTPN6 Antibody Application Details Handling Images back to top
|Alternative Name||PTPN6 (PTPN6 Antibody Abstract)|
SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosinephosphatase (PTP) predominantly expressed in hematopoietic cells, and regulatesmultiple signaling pathways involved in hematopoietic cell growth, differentiation andactivation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosolto plasma membrane and bind to the tyrosine-phosphorylated receptors through theirSH2 domains, becoming activated in the process (2). SHP-1 has been involved in thenegative regulation of various growth promoting receptors including receptor tyrosinekinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine basedreceptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5). Alternate Names: anti-70 kda SHP 1L protein antibody,anti-kda SHP1L protein antibody, anti-P antibody,anti-PH antibody, anti-matopoietic cell phosphatase antibody, anti-matopoietic cellprotein-tyrosine phosphatase antibody, anti-TP 1C antibody, anti-TP1C antibody,anti-otein tyrosine phosphatase 1C antibody, anti-protein tyrosine phosphatase nonreceptor type 6 antibody, anti-protein tyrosine phosphatase SHP 1 antibody, anti-proteintyrosine phosphatase SHP1 antibody,PTP 1C antibody, anti-PTP1C antibody,anti-PTPN 6 antibody, anti-PTPN6 antibody, anti-SH PTP 1 antibody, anti-SH PTP1antibody, anti-SHP 1 antibody, anti-SHP 1L antibody, anti-SHP1L antibody, anti-SHPTP1 antibody, anti-SHPTP1 antibody, anti-Tyrosine protein phosphatase non receptor type6 antibody.
Gene Symbol: PTPN6
|Gene ID||5777, 23957, 116689|
|Pathways||JAK-STAT Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway|
Application DetailsProduct Details anti-PTPN6 Antibody Target Details PTPN6 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 65kDa.
Recommended dilutions: Flow Cytometry 1:20, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000
Protocol specific for PTPN6 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
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