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|Application / Reactivity||Human|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|Enzyme Immunoassay (EIA)||12 Antibodies|
|Flow Cytometry (FACS)||18 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunochromatography (IC)||7 Antibodies|
|Immunocytochemistry (ICC)||38 Antibodies|
|Immunodiffusion (ID)||1 Antibodies|
|Immunofluorescence (IF)||72 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||196 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||61 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN358234) Images
|Specificity||This antibody detects STAT3 pTyr705.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr705 of human STAT3.|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN358234) Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
|Background||STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3|
|Molecular Weight||88068 Da|
|Gene ID||6774, 9606|
|Research Area||Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN358234) Images back to top
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN358234) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-STAT3 Antibody (ABIN358234)Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Schick, Oakeley, Hynes et al.: "TEL/ETV6 is a signal transducer and activator of transcription 3 (Stat3)-induced repressor of Stat3 activity." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38787-96, 2004 (PubMed).
Ivanova, Ivanov, Zhang et al.: "STRA13 interacts with STAT3 and modulates transcription of STAT3-dependent targets." in: Journal of molecular biology, Vol. 340, Issue 4, pp. 641-53, 2004 (PubMed).
Park, Seo, Jung et al.: "Herpesvirus saimiri STP A11 protein interacts with STAT3 and stimulates its transcriptional activity." in: Biochemical and biophysical research communications, Vol. 320, Issue 1, pp. 279-85, 2004 (PubMed).
Wang, Wang, Zhang et al.: "Analysis of the activation status of Akt, NFkappaB, and Stat3 in human diffuse gliomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 8, pp. 941-51, 2004 (PubMed).
Kato, Sakamoto, Kutsuna et al.: "Proteolytic conversion of STAT3alpha to STAT3gamma in human neutrophils: role of granule-derived serine proteases." in: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31076-80, 2004 (PubMed).
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