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anti-Human STAT3 antibody for Enzyme Immunoassay

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
pSer727
107
94
83
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
519
248
192
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
452
97
34
4
1
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
7
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
484
233
206
74
69
64
46
25
23
13
12
11
9
7
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN358233
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN358234 EIA IHC (p) WB Rabbit Ig pTyr705 Log in to see Polyclonal 5
0.0008833851 ABIN374087 EIA IF IHC (p) WB Goat C-Term, Isoform 1 Log in to see Polyclonal 1
0.0008833851 ABIN357083 EIA WB Rabbit Ig pSer727 Log in to see Polyclonal
0.0008833851 ABIN271827 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008833851 ABIN126898 EIA IHC (fro) IF IP WB Mouse IgG1 pTyr705 Log in to see 9-00E-012
0.0008833851 ABIN126896 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pTyr705 Log in to see 9-00E-012
0.0008833851 ABIN126899 EIA IHC (fro) WB Mouse IgG1 phosphorylated Log in to see 23G5
0.0008833851 ABIN1109152 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3B5 2
0.0008833851 ABIN1109151 EIA IHC (p) WB Mouse IgG1 Log in to see 7G3H4 2
0.0008833851 ABIN1109150 EIA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008833851 ABIN641240 CA DB EIA IA ISt ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-STAT3 antibody for Enzyme Immunoassay

Similar anti-STAT3 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 199 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Gel Shift (GS) 2 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunodiffusion (ID) 1 Antibodies
Immunofluorescence (IF) 72 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 196 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 61 Antibodies
Immunoprecipitation (IP) 53 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pSer727
(107), (94), (83), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(519), (248), (192), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(452), (97), (34), (4), (1), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (7), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(484), (233), (206), (74), (69), (64), (46), (25), (23), (13), (12), (11), (9), (7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN358233) Images
Specificity This antibody detects STAT3 pSer727.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
Isotype Ig

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN358233) Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3
Molecular Weight 88068 Da
Gene ID 6774, 9606
UniProt P40763
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN358233) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN358233) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-STAT3 Antibody (ABIN358233)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Background publications

Schick, Oakeley, Hynes, Badache: "TEL/ETV6 is a signal transducer and activator of transcription 3 (Stat3)-induced repressor of Stat3 activity." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38787-96, 2004

Wang, Wang, Zhang, Huang, Liao, Fuller: "Analysis of the activation status of Akt, NFkappaB, and Stat3 in human diffuse gliomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 8, pp. 941-51, 2004

Kato, Sakamoto, Kutsuna, Kimura-Eto, Hato, Kitagawa: "Proteolytic conversion of STAT3alpha to STAT3gamma in human neutrophils: role of granule-derived serine proteases." in: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31076-80, 2004

Ivanova, Ivanov, Zhang, Ivanov, Timofeeva, Lerman: "STRA13 interacts with STAT3 and modulates transcription of STAT3-dependent targets." in: Journal of molecular biology, Vol. 340, Issue 4, pp. 641-53, 2004

Park, Seo, Jung, Choe: "Herpesvirus saimiri STP A11 protein interacts with STAT3 and stimulates its transcriptional activity." in: Biochemical and biophysical research communications, Vol. 320, Issue 1, pp. 279-85, 2004

Images

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Supplier Images
 image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (ABIN358233) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
 image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (ABIN358233) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody (Image 2)