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STAT3 antibody (pSer727)

Name: STAT3 antibody (pSer727)
SKU: ABIN5557530
Availability: InStock

This Rabbit Monoclonal antibody specifically detects STAT3 in WB, IHC (p), FACS and IF (cc). It exhibits reactivity toward Human, Mouse and Rat.

Lane 1: HeLa lysates, Lane 2: NIH/3T3 lysates, probed with STAT3(S727) (4G1 ) Monoclonal Antibody at 1:1000 overnight at 4˚C. (STAT3 antibody (pSer727))

Paraformaldehyde-fixed, paraffin embedded rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (STAT3 antibody (pSer727))

IHC (p)

HeLa cells were fixed in paraformaldehyde, permeabilized with 0. (STAT3 antibody (pSer727))

IF (cc)

A431 cells were fixed in paraformaldehyde, permeabilized with 0. (STAT3 antibody (pSer727))

IF (cc)

NIH/3T3 cells were fixed in paraformaldehyde, permeabilized with 0. (STAT3 antibody (pSer727))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded rat liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (STAT3 antibody (pSer727))

IHC (p)

7 images

Catalog No. ABIN5557530

$490.77

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 8 to 12 Business Days

Quick Overview for STAT3 antibody (pSer727) (ABIN5557530)

Target

See all STAT3 Antibodies

STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Monoclonal

Conjugate

This STAT3 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Clone

4G1

Discover our top product STAT3 Primary Antibody

Binding Specificity
93

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pSer727

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

Synthetic peptide derived from human STAT3(S727), around 700-750aa (phospho S727).

Isotype

IgG
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
KO Validated STAT3 Reactivity: Human WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 640-770) antibody
STAT3 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody
STAT3 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
STAT3 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
STAT3 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 233-330) antibody
STAT3 Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 670-769) antibody
STAT3 Reactivity: Human WB, ELISA, IF, PLA Host: Mouse Monoclonal 4D6 unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
Verified STAT3 Reactivity: Human, Mouse IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
STAT3 Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IP, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody
STAT3 Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name

STAT3

Background

Synonyms: APRF, HIES, ADMIO, Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3
Background: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).

Gene ID

6774

UniProt

P40763

Pathways

JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor