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|Application / Reactivity||Rat (Rattus)|
|Chromatin Immunoprecipitation (ChIP)||2 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||39 Antibodies|
|Gel Shift (GS)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||37 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||2 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||27 Antibodies|
|Immunohistochemistry (IHC)||95 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||54 Antibodies|
|Immunoprecipitation (IP)||57 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||209 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of STAT3 protein. (region surrounding Thr721)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
|Background||Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3|
|Molecular Weight||approx. 88 kDa|
|Research Area||Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
ELISA: 1:10000approx. 1:20000. WB: 1:500approx. 1:1000. IHC: 1:50approx. 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling back to top