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anti-Rat (Rattus) STAT3 antibody for Immunocytochemistry

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
AA 751-770, C-Term
107
93
84
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
519
248
191
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
452
97
34
4
1
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
7
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
484
233
206
74
69
64
45
25
23
13
12
12
9
7
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN3044166
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010113637 ABIN2855865 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010113637 ABIN3044335 ICC IHC (p) WB Rabbit IgG AA 686-698, C-Term Log in to see Polyclonal
0.0010113637 ABIN256912 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr705 Log in to see EP2147Y
0.0010113637 ABIN1860649 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 135-279 Log in to see Polyclonal
0.0010113637 ABIN1860651 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 656-768 Log in to see Polyclonal
0.0010113637 ABIN343631 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010113637 ABIN2987277 ICC IF IHC WB Rabbit IgG pTyr705 Log in to see Polyclonal
0.0010113637 ABIN2987403 ICC IF IHC WB Rabbit IgG pSer727 Log in to see Polyclonal
0.0010113637 ABIN1112929 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010113637 ABIN1854975 ICC IF WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010113637 ABIN2969383 ICC FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010113637 ABIN3032574 ICC IHC (p) WB Rabbit IgG AA 686-698 Log in to see Polyclonal
0.0010113637 ABIN3032575 ICC IHC (p) WB Rabbit IgG AA 751-770 Log in to see Polyclonal
0.0010113637 ABIN1579701 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010113637 ABIN2970895 ICC IF IP IHC WB Rabbit Log in to see Polyclonal
0.0010113637 ABIN1689945 ICC IHC (p) WB Rabbit Log in to see Polyclonal

Similar anti-STAT3 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 38 Antibodies 199 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 12 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 86 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Western Blotting (WB) 148 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope AA 751-770, C-Term
(107), (93), (84), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(519), (248), (191), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(452), (97), (34), (4), (1), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (7), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(484), (233), (206), (74), (69), (64), (45), (25), (23), (13), (12), (12), (9), (7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling Images
Sequence QFESLTFDME LTSECATSPM
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: signal transducer and activator of transcription 3(acute-phase response factor)
Protein Name: Signal transducer and activator of transcription 3
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human STAT3(751-770aa QFESLTFDMELTSECATSPM), different from the related rat and mouse sequences by one amino acid.
Isotype IgG

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription factor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member of the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression of STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at its amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl terminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered glycolytic and oxidative phosphorylation activities characteristic of cancer cells.
UniProt P40763
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (AA 751-770) (ABIN3044166) Anti-STAT3 antibody, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Bra...
Immunohistochemistry (IHC) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (AA 751-770) (ABIN3044166) Anti-STAT3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue