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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||38 Antibodies||199 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||12 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||17 Antibodies|
|Immunofluorescence (IF)||30 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||86 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies|
|Immunoprecipitation (IP)||29 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies|
|Western Blotting (WB)||148 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Epitope||AA 751-770, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 3(STAT3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: signal transducer and activator of transcription 3(acute-phase response factor)
Protein Name: Signal transducer and activator of transcription 3
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human STAT3(751-770aa QFESLTFDMELTSECATSPM), different from the related rat and mouse sequences by one amino acid.|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
|Background||Stat3(Signal transducer and activator of transcription 3), also called APRF, is a transcription factor which in humans is encoded by theSTAT3 gene. The protein encoded by this gene is a member of the STAT protein family. The Stat3 gene is mapped on 17q21.2. Binding of IL5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Coexpression of STAT3-beta inhibits the transactivation potential of STAT3. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The transcriptional coactivator p300 interacted physically with STAT3 at its amino terminus in a cytokine stimulation-independent manner, and with SMAD1 at its carboxyl terminus in a cytokine stimulation-dependent manner. In many human cancers and transformed cell lines, STAT3 is persistently activated, and in cell culture, active STAT3 is either required for transformation, enhances transformation, or blocks apoptosis. Mitochondrial STAT3 sustained altered glycolytic and oxidative phosphorylation activities characteristic of cancer cells.|
|Research Area||Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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