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anti-Human STAT4 antibody for Western Blotting

Recommended STAT4 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
  • SLEB11
Epitope
Internal Region, AA 369-383
70
29
15
13
13
10
7
6
6
6
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
270
104
79
8
8
4
3
2
2
2
1
Host
Goat
181
71
27
2
2
Clonality
Polyclonal
Conjugate
This STAT4 antibody is un-conjugated
9
9
9
8
7
6
4
3
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
203
106
83
46
37
36
25
22
7
6
4
3
3
2
1
Supplier
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Catalog No. ABIN499125
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476677 ABIN499124 EIA WB Goat Internal Region, AA 613-627 Log in to see Polyclonal 1
0.13476677 ABIN2777897 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.13476677 ABIN117931 EIA IP WB Rabbit AA 730-749 Log in to see Polyclonal 8
0.13476677 ABIN411597 WB Rabbit IgG Log in to see Polyclonal
0.13476677 ABIN1531976 IHC ELISA WB Rabbit IgG pTyr693 Log in to see Polyclonal
0.13476677 ABIN657644 IF IHC (p) WB Rabbit Ig AA 666-695, C-Term Log in to see Polyclonal
0.13476677 ABIN2464866 ELISA WB Goat Internal Region Log in to see Polyclonal
0.13476677 ABIN1533011 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476677 ABIN2464865 ELISA WB Goat Internal Region Log in to see Polyclonal
0.13476677 ABIN781830 EIA IF IHC (p) IP WB Mouse IgG1 Log in to see 1C2-1C12
0.13476677 ABIN3043099 WB Rabbit IgG AA 1-17, N-Term Log in to see Polyclonal
0.13476677 ABIN783862 WB Rabbit Log in to see Polyclonal
0.13476677 ABIN2705262 IHC WB Rabbit C-Term, pTyr693 Log in to see Polyclonal
0.13476677 ABIN2452527 IHC WB Mouse IgG1 AA 633-652 Log in to see 2A2
0.13476677 ABIN1870645 IHC WB Rabbit IgG pTyr693 Log in to see Polyclonal
0.13476677 ABIN2460707 IHC ELISA WB Rabbit Log in to see Polyclonal
0.13476677 ABIN2506005 IHC WB Rabbit IgG Tyr693 Log in to see Polyclonal
0.13476677 ABIN2506006 IHC WB Rabbit IgG pTyr693 Log in to see Polyclonal
0.13476677 ABIN1874965 WB Rabbit IgG Log in to see Polyclonal
0.13476677 ABIN2650957 WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-STAT4 antibody for Western Blotting

Similar anti-STAT4 Antibodies

Application / Reactivity Human
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 108 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Intracellular Staining (ICS) 7 Antibodies
Luminex Assay (LMNX) 6 Antibodies
Western Blotting (WB) 193 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
Epitope Internal Region, AA 369-383
(70), (29), (15), (13), (13), (10), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(270), (104), (79), (8), (8), (4), (3), (2), (2), (2), (1)
Host Goat
(181), (71), (27), (2), (2)
Clonality
Polyclonal
Conjugate This STAT4 antibody is un-conjugated
(9), (9), (9), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(203), (106), (83), (46), (37), (36), (25), (22), (7), (6), (4), (3), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-STAT4 Antibody

Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN499125) Images
Sequence TLSNRRFVLC GTNVK
Specificity Recognizes STAT4 (aa 369-383).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region (aa 369-383) of the protein sequence according to Human STAT4 (NP_003142.1). Genename: STAT4

Target Details STAT4

Product Details anti-STAT4 Antibody Application Details Handling References for anti-STAT4 Antibody (ABIN499125) Images back to top
Antigen
Alternative Name STAT4 (STAT4 Antibody Abstract)
Background The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4
Gene ID 6775
NCBI Accession NP_003142
UniProt Q14765
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT4 Antibody Target Details STAT4 Handling References for anti-STAT4 Antibody (ABIN499125) Images back to top
Application Notes Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.5-1.0 μg/mL. This antibody detects a band of approx. 85 kDa in transfected HEK293transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details References for anti-STAT4 Antibody (ABIN499125) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-STAT4 Antibody (ABIN499125)

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling Images back to top
Background publications

Good, Thieu, Mathur, Yu, Stritesky, Yeh, OMalley, Perumal, Kaplan: "Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 6, pp. 3839-47, 2009

Boisson, Annat, Aimard, Pequignot, Grivet, Devic: "[Treatment of a case of grave orthostatic hypotension (Shy-Drager's syndrome) by an association of L-dopa and mono-amine-oxidase inhibitor (author's transl)]." in: La Nouvelle presse médicale, Vol. 6, Issue 41, pp. 3839-41, 1978

Images

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN499125) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT4 antibody (Signal Transducer and Activator of Transcription 4) (Internal Region) (ABIN499125) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human STAT4 with C-termin...