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anti-Sheep (Ovine) STAT5A antibody for Intracellular Staining

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
pTyr694
88
51
44
16
16
13
10
6
6
6
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
441
224
196
35
23
19
17
17
7
4
3
1
1
1
1
1
1
Host
Mouse
325
120
14
Clonality
Monoclonal
Conjugate
This STAT5A antibody is conjugated to PerCP-Cy5.5
12
12
10
8
7
7
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
352
207
144
86
83
71
38
31
26
17
10
9
7
7
6
4
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1177246
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177247 ICS PE-Cy7 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177248 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177254 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 8
1 ABIN1177249 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177250 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177251 ICS Pacific Blue Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177252 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5
1 ABIN1177253 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5

Top referenced anti-STAT5A antibody for Intracellular Staining

Similar anti-STAT5A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Sheep (Ovine)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 335 Antibodies 173 Antibodies 147 Antibodies 14 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 6 Antibodies
Intracellular Staining (ICS) 11 Antibodies 9 Antibodies 9 Antibodies 9 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 36 Antibodies 21 Antibodies 17 Antibodies 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pTyr694
(88), (51), (44), (16), (16), (13), (10), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(441), (224), (196), (35), (23), (19), (17), (17), (7), (4), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(325), (120), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is conjugated to PerCP-Cy5.5
(12), (12), (10), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(352), (207), (144), (86), (83), (71), (38), (31), (26), (17), (10), (9), (7), (7), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN1177246) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Phosphorylated Stat5 Peptide
Clone 47-Stat5
Isotype IgG1, kappa

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN1177246) Images back to top
Antigen
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.
Research Area Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN1177246) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I).
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN1177246) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

References for anti-STAT5A Antibody (ABIN1177246)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Johnston, Choi, Diamond, Yang, Crotty: "STAT5 is a potent negative regulator of TFH cell differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 2, pp. 243-50, 2012

Suni, Maino: "Flow cytometric analysis of cell signaling proteins." in: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, 2011

Prlic, Bevan: "Exploring regulatory mechanisms of CD8+ T cell contraction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 43, pp. 16689-94, 2008

Riou, Yassine-Diab, Van grevenynghe, Somogyi, Greller, Gagnon, Gimmig, Wilkinson, Shi, Cameron, Campos-Gonzalez, Balderas, Kelvin, Sekaly, Haddad: "Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 79-91, 2007

Van De Wiele, Marino, Murray, Vo, Whetsell, Teague: "Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, 2004

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000

Imada, Leonard: "The Jak-STAT pathway." in: Molecular immunology, Vol. 37, Issue 1-2, pp. 1-11, 2000

Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, 1998

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994