STAT5A antibody (C-Term)

Catalog No. ABIN5539581

The Goat Polyclonal anti-STAT5A antibody is suitable to detect STAT5A in samples from Human and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for STAT5A antibody (C-Term) (ABIN5539581)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-STAT5A Antibody

Binding Specificity: C-Term

Purpose: STAT5A

Sequence: DSRLSPPAGL FTSAR

Specificity: This antibody is not expected to cross-react with STAT5B.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line NIH3T3 and in Mouse Lymph node lysates and approx. 90-100 kDa in lysates of cell line K562 (calculated MW of 90.6 kDa according to Human NP_003143.2 and 90.8 kDa according to Mouse NP_0013496

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A

Background: STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5

Gene ID: 6776, 20850, 24918

NCBI Accession: NP_003143

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation