TDP1 ELISA Kit (Tyrosyl-DNA Phosphodiesterase 1)

Details for Product TDP1 ELISA Kit No. ABIN5564588, Supplier: Log in to see
Antigen
  • tdp1
  • MGC83708
  • TDP1
  • F8M21.60
  • F8M21_60
  • tyrosyl-DNA phosphodiesterase 1
  • 2810481F14Rik
  • 4921509N21Rik
  • AI838772
  • AW493413
  • E430034L06Rik
  • MLZ-501
  • SCAN1
  • tyrosyl-DNA Phosphodiesterase (Tdp1)
  • tyrosyl-DNA phosphodiesterase (Tdp1)
  • tyrosyl-DNA phosphodiesterase 1
  • Tc00.1047053511191.10
  • Tc00.1047053506619.40
  • TDP1
  • tdp1
  • PTRG_11198
  • MCYG_02121
  • MGYG_03035
  • Tdp1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
1
Method Type
Sandwich ELISA
Application
ELISA
Options
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Brand AssayMax™
Sample Type Cell Culture Cells, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Material not included Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
Alternative Name TDP1 (TDP1 ELISA Kit Abstract)
Gene ID 55775
UniProt Q9NUW8
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Assay Time 4 h
Plate Pre-coated
Calculation of Results
  • Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
  • To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
  • Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
Restrictions For Research Use only
Handling Advice This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
Storage 4 °C,-20 °C