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anti-Human ARAF antibody for Immunocytochemistry

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • 1200013E08Rik
  • A-RAF
  • a-raf
  • A-Raf
  • araf
  • ARAF
  • ARAF1
  • araf1
  • Araf1
  • AW495444
  • pks2
  • PKS2
  • rafa1
  • RAFA1
  • wu:fk45h07
  • zgc:92074
Epitope
C-Term
21
20
18
15
14
13
13
9
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
129
66
62
3
3
2
2
1
1
Host
Rabbit
108
23
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
86
52
33
29
19
10
4
3
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2855039
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN1832873 ICC IHC (p) WB Rabbit IgG AA 371-554 Log in to see Polyclonal
0.0009965416 ABIN2157019 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.0009965416 ABIN2883484 ICC IHC ELISA WB Rabbit IgG pTyr302 Log in to see Polyclonal
0.0009965416 ABIN443011 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0009965416 ABIN4277010 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-ARAF Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 18 Antibodies 51 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 6 Antibodies
Immunofluorescence (IF) 7 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 31 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 32 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope C-Term
(21), (20), (18), (15), (14), (13), (13), (9), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(129), (66), (62), (3), (3), (2), (2), (1), (1)
Host Rabbit
(108), (23)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(86), (52), (33), (29), (19), (10), (4), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Cross-Reactivity Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine)
Cross-Reactivity (Details) Pig (99 %), Rat (99 %), Zebrafish (85 %), Bovine (99 %)
Characteristics Rabbit Polyclonal antibody to A-RAF (v-raf murine sarcoma 3611 viral oncogene homolog)
A-RAF antibody [C1C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
Isotype IgG

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name A-RAF (ARAF Antibody Abstract)
Background This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.

Molecular Weight 68 kDa
Gene ID 369
Pathways MAPK Signaling, RTK Signaling

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Format Liquid
Concentration 0.85 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (C-Term) (ABIN2855039) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF, ...
Western Blotting (WB) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (C-Term) (ABIN2855039) WB Image Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE antibody diluted a...
Western Blotting (WB) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (C-Term) (ABIN2855039) WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE antibody diluted...
Immunohistochemistry (IHC) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (C-Term) (ABIN2855039) IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ...