anti-Human ARAF antibody for Immunoprecipitation

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • v-raf murine sarcoma 3611 viral oncogene homolog
  • araf
  • ARAF
  • LOC100340437
  • araf-a
  • araf-b
  • Araf
Epitope
AA 183-199
23
20
16
15
14
12
10
9
8
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
146
81
74
6
6
6
2
2
2
2
1
Host
Mouse
121
25
Clonality
Monoclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
98
52
39
29
28
13
6
2
2
2
1
1
1
Options
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Catalog No. ABIN967727
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1976720 IP Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN1976721 IP WB Rabbit IgG AA 150-200 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope AA 183-199
(23), (20), (16), (15), (14), (12), (10), (9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(146), (81), (74), (6), (6), (6), (2), (2), (2), (2), (1)
Host Mouse
(121), (25)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(98), (52), (39), (29), (28), (13), (6), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling References for anti-ARAF antibody (ABIN967727) Images
Cross-Reactivity Human, Chicken, Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse A-Raf aa. 183-199
Clone 1-A
Isotype IgG1

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling References for anti-ARAF antibody (ABIN967727) Images back to top
Antigen
Alternative Name A-Raf (ARAF Antibody Abstract)
Background A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.
Molecular Weight 68 kDa
Pathways MAPK Signaling, RTK Signaling, Hepatitis C

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling References for anti-ARAF antibody (ABIN967727) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details References for anti-ARAF antibody (ABIN967727) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-ARAF antibody (ABIN967727)

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling Images back to top
Background publications

Raabe, Rapp: "KSR--a regulator and scaffold protein of the MAPK pathway." in: Science's STKE : signal transduction knowledge environment, Vol. 2002, Issue 136, pp. pe28, 2002

Product cited in:

Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, 2002

Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, 1990

Rapp, Heidecker, Huleihel, Cleveland, Choi, Pawson, Ihle, Anderson: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, 1989

Huleihel, Goldsborough, Cleveland, Gunnell, Bonner, Rapp: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, 1987

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling References for anti-ARAF antibody (ABIN967727) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (ABIN967727) Western blot analysis of A-Raf on SKN, a human neuroblastoma, cell lysate. Lane 1: 1:...
 image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (ABIN967727) Immunoflourescence staining of rabbit kidney.
 image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (ABIN967727) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (Image 3)
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (ABIN967727) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (Image 4)