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anti-Mouse (Murine) ARAF antibody for Immunohistochemistry

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • 1200013E08Rik
  • A-RAF
  • a-raf
  • A-Raf
  • araf
  • ARAF
  • ARAF1
  • araf1
  • Araf1
  • AW495444
  • pks2
  • PKS2
  • rafa1
  • RAFA1
  • wu:fk45h07
  • zgc:92074
Epitope
pTyr302
20
20
18
15
14
13
13
9
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
131
68
62
3
3
2
2
1
1
Host
Rabbit
110
23
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
88
51
33
30
22
11
6
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1531487
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22865479 ABIN1532501 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22865479 ABIN2162676 IHC ELISA WB Rabbit IgG Tyr301, Tyr302 Log in to see Polyclonal
0.0011340706 ABIN967727 IF IP IHC WB Mouse IgG1 AA 183-199 Log in to see 1-A 5
0.0011340706 ABIN1450419 IHC ELISA Rabbit IgG pSer299, Internal Region Log in to see Polyclonal 1
0.0011340706 ABIN3183344 ELISA IHC WB Rabbit IgG Ser155 Log in to see Polyclonal
0.0011340706 ABIN3182781 ELISA IF IHC WB Rabbit IgG pTyr302 Log in to see Polyclonal
0.0011340706 ABIN3182911 ELISA IHC Rabbit IgG pSer299 Log in to see Polyclonal
0.0011340706 ABIN443011 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN408002 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN2162677 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1574357 IF IHC ELISA WB Rabbit IgG pTyr302 Log in to see Polyclonal
0.0011340706 ABIN1574358 IHC ELISA WB Rabbit IgG Tyr301, Tyr302 Log in to see Polyclonal
0.0011340706 ABIN2133974 IHC ELISA Rabbit IgG pSer299 Log in to see Polyclonal
0.0011340706 ABIN2133975 IF IHC ELISA WB Rabbit IgG pTyr301 Log in to see Polyclonal
0.0011340706 ABIN3060304 IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-ARAF antibody for Immunohistochemistry

Similar anti-ARAF Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 17 Antibodies 18 Antibodies 51 Antibodies
Immunofluorescence (IF) 5 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 13 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 29 Antibodies 34 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope pTyr302
(20), (20), (18), (15), (14), (13), (13), (9), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(131), (68), (62), (3), (3), (2), (2), (1), (1)
Host Rabbit
(110), (23)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(88), (51), (33), (30), (22), (11), (6), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Specificity A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A- RAF only when phosphorylated at Tyr302.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.
Isotype IgG

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name A-RAF (ARAF Antibody Abstract)
Background Molecular weight of target protein: 67 kDa
Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto- oncogene Pks, ARAF, ARAF1, PKS, PKS2
Molecular Weight 67 kDa
Gene ID 369
OMIM 311010
UniProt P10398
Pathways MAPK Signaling, RTK Signaling

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.225936, Hs.446641 (NCBI Gene Symbol: ARAF)

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (pTyr302) (ABIN1531487) Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', usin...
Immunohistochemistry (IHC) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (pTyr302) (ABIN1531487) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RA...
Immunofluorescence (IF) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (pTyr302) (ABIN1531487) Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. Th...