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anti-Human BAD antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
AA 140-168
51
45
41
37
36
36
34
27
27
23
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
710
364
208
9
5
4
2
2
1
Host
Rabbit
903
48
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
509
418
415
279
66
59
46
23
8
4
4
3
2
2
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN674709
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088963145 ABIN1701917 IF (p) Cy3 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1696480 IF (p) Alexa Fluor 555 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1698291 IF (p) Alexa Fluor 647 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1705539 IF (p) Cy5.5 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1703728 IF (p) Cy5 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1707497 IF (p) Cy7 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1709308 IF (p) FITC Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN2810152 IF (p) Alexa Fluor 594 Rabbit IgG pSer118 Log in to see Polyclonal
0.00088963145 ABIN2810146 IF (p) Alexa Fluor 594 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN2810148 IF (p) Alexa Fluor 594 Rabbit IgG pSer75 Log in to see Polyclonal
0.00088963145 ABIN2810153 IF (p) Alexa Fluor 594 Rabbit IgG pSer134 Log in to see Polyclonal
0.00088963145 ABIN2810154 IF (p) Alexa Fluor 594 Rabbit IgG pSer99 Log in to see Polyclonal
0.00088963145 ABIN1694651 IF (p) Alexa Fluor 488 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN1692837 IF (p) Alexa Fluor 350 Rabbit IgG pSer91 Log in to see Polyclonal
0.00088963145 ABIN876973 IF (p) Alexa Fluor 555 Rabbit IgG pSer75 Log in to see Polyclonal
0.00088963145 ABIN877387 IF (p) Alexa Fluor 350 Rabbit IgG pSer99 Log in to see Polyclonal
0.00088963145 ABIN877384 IF (p) Alexa Fluor 555 Rabbit IgG pSer99 Log in to see Polyclonal
0.00088963145 ABIN877385 IF (p) Alexa Fluor 647 Rabbit IgG pSer99 Log in to see Polyclonal
0.00088963145 ABIN877383 IF (p) Alexa Fluor 488 Rabbit IgG pSer99 Log in to see Polyclonal
0.00088963145 ABIN876972 IF (p) Alexa Fluor 350 Rabbit IgG pSer75 Log in to see Polyclonal

Similar anti-BAD Antibodies

Application / Reactivity Mouse (Murine) Human
Dot Blot (DB) 132 Antibodies 250 Antibodies
ELISA 163 Antibodies 364 Antibodies
Flow Cytometry (FACS) 7 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 6 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 135 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 31 Antibodies
Western Blotting (WB) 177 Antibodies
Cellular Assay (CA) 1 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope AA 140-168
(51), (45), (41), (37), (36), (36), (34), (27), (27), (23), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(710), (364), (208), (9), (5), (4), (2), (2), (1)
Host Rabbit
(903), (48), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(509), (418), (415), (279), (66), (59), (46), (23), (8), (4), (4), (3), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-BAD Antibody

Target Details BAD Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Bad
Isotype IgG

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion


Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6
Gene ID 572
UniProt Q92934
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 140-168) (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 140-168) (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
'Independent Validation' Badge
Lot Number 090915
Method validated Western Blotting
Positive Control MCF7 cells
Negative Control C6/36 cells (non-reactive species)
Notes A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Primary Antibody
  • Antigen: BCL2-Associated Agonist of Cell Death (BAD)
  • Catalog number: ABIN674709
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 090915
  • Antibody Dilution: 1:200
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
Protocol
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...