anti-Human BAD antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-antagonist of cell death b
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitope
AA 140-168
60
58
52
49
47
45
43
29
22
21
21
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
19
19
19
15
15
12
10
9
8
8
7
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
779
434
265
19
15
8
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
957
49
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
65
64
64
58
58
58
6
6
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
527
464
396
295
113
78
53
29
13
12
6
5
4
3
2
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN674709
$ 295.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.569977 ABIN213744 IHC (p) WB Rabbit IgG pSer112 Log in to see Polyclonal
11.569977 ABIN214418 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
11.569977 ABIN498774 IHC (p) WB Rabbit pSer112 Log in to see Polyclonal
11.569977 ABIN461986 IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001
11.569977 ABIN498190 IF IHC (p) WB Rabbit Log in to see Polyclonal
11.569977 ABIN498864 IHC (p) Rabbit Log in to see Polyclonal
11.569977 ABIN4282857 IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
11.569977 ABIN4282865 IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001
11.569977 ABIN498091 IHC (p) WB Rabbit Log in to see Polyclonal
11.569977 ABIN756562 IF (p) IHC (p) WB Rabbit IgG pSer118 Log in to see Polyclonal
11.569977 ABIN682828 IF (p) IHC (p) WB Rabbit IgG pSer75 Log in to see Polyclonal
11.569977 ABIN4282853 ICC IF IHC IHC (p) WB Rabbit pSer155 Log in to see Polyclonal
11.569977 ABIN4282868 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
11.569977 ABIN5557534 IHC (p) WB Rabbit IgG Log in to see 5D4
1 ABIN318334 IHC (p) WB Rabbit pSer136, pSer99 Log in to see Polyclonal 1
1 ABIN358087 EIA IHC (p) WB Rabbit Ig pSer99 Log in to see Polyclonal 5
1 ABIN389519 IHC (p) WB Rabbit Ig pSer134 Log in to see Polyclonal 2
1 ABIN389521 DB IF IHC (p) Rabbit Ig pSer99 Log in to see Polyclonal 2
1 ABIN389518 IHC (p) WB Rabbit Ig pSer118 Log in to see Polyclonal 2
1 ABIN389520 IHC (p) WB Rabbit Ig pSer75 Log in to see Polyclonal 2

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope AA 140-168
(60), (58), (52), (49), (47), (45), (43), (29), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (15), (15), (12), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(779), (434), (265), (19), (15), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(957), (49), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(65), (64), (64), (58), (58), (58), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(527), (464), (396), (295), (113), (78), (53), (29), (13), (12), (6), (5), (4), (3), (2), (1), (1), (1)
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Bad
Isotype IgG

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

Gene ID 572
UniProt Q92934
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Image provided by the Independent Validation Program (badge number 29802). Lane 1: MC...
'Independent Validation' Badge
Lot Number 090915
Method validated Western Blotting
Positive Control MCF7 cells
Negative Control C6/36 cells (non-reactive species)
Notes A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Primary Antibody
  • Antigen: BCL2-Associated Agonist of Cell Death (BAD)
  • Catalog number: ABIN674709
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 090915
  • Antibody Dilution: 1:200
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
Protocol
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...