You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human BCL2 antibody for Intracellular Staining

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
pSer70
76
70
53
50
48
44
23
23
19
17
15
14
14
13
13
13
13
12
12
9
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
673
200
151
17
9
7
4
3
2
2
1
1
Host
Mouse
494
239
19
5
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
38
33
28
24
19
14
11
10
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), Western Blotting (WB)
480
275
241
145
138
138
113
67
49
42
33
11
10
10
7
7
5
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1177235
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN1177234 ICS Alexa Fluor 647 Mouse IgG1 pSer70 Log in to see N46-467 5
0.0008676689 ABIN1177233 ICS PE Mouse IgG1 pSer70 Log in to see N46-467 5

Top referenced anti-BCL2 antibody for Intracellular Staining

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 246 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 116 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunofluorescence (IF) 146 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 240 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 112 Antibodies
Immunoprecipitation (IP) 132 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope pSer70
(76), (70), (53), (50), (48), (44), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(673), (200), (151), (17), (9), (7), (4), (3), (2), (2), (1), (1)
Host Mouse
(494), (239), (19), (5), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(38), (33), (28), (24), (19), (14), (11), (10), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), Western Blotting (WB)
(480), (275), (241), (145), (138), (138), (113), (67), (49), (42), (33), (11), (10), (10), (7), (7), (5), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1177235) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Peptide
Clone N46-467
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background The clone N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50
Molecular Weight ~28 kDa
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Application Notes IHC(F): Antigen retrieval required
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-BCL2 Antibody (ABIN1177235)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Geng, Tang, Li, Yang, Choi, Kazim, Zhang: "Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, 2011

Sarkar, Korolchuk, Renna, Imarisio, Fleming, Williams, Garcia-Arencibia, Rose, Luo, Underwood, Kroemer, OKane, Rubinsztein: "Complex inhibitory effects of nitric oxide on autophagy." in: Molecular cell, Vol. 43, Issue 1, pp. 19-32, 2011

Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, 2001

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, 1998