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anti-Human BCL2 antibody for Intracellular Staining

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
pSer70
166
73
58
57
44
33
25
25
24
18
15
15
15
13
13
13
12
12
11
9
7
5
5
5
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
992
259
211
54
28
22
19
18
5
4
4
2
2
2
2
1
Host
Mouse
547
512
20
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
47
47
41
38
24
23
20
15
13
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), Western Blotting (WB)
798
381
358
314
302
300
228
187
169
81
42
13
12
12
10
9
9
5
2
1
1
1
1
1
Options
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Catalog No. ABIN1177235
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177234 ICS Alexa Fluor 647 Mouse IgG1 pSer70 Log in to see N46-467 5
1 ABIN1177233 ICS PE Mouse IgG1 pSer70 Log in to see N46-467 5

Top referenced anti-BCL2 antibody for Intracellular Staining

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 350 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 286 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 9 Antibodies
Immunocytochemistry (ICC) 173 Antibodies
Immunofluorescence (IF) 298 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 68 Antibodies
Immunohistochemistry (IHC) 289 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 166 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 350 Antibodies
Immunoprecipitation (IP) 213 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope pSer70
(166), (73), (58), (57), (44), (33), (25), (25), (24), (18), (15), (15), (15), (13), (13), (13), (12), (12), (11), (9), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(992), (259), (211), (54), (28), (22), (19), (18), (5), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(547), (512), (20), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(47), (47), (41), (38), (24), (23), (20), (15), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), Western Blotting (WB)
(798), (381), (358), (314), (302), (300), (228), (187), (169), (81), (42), (13), (12), (12), (10), (9), (9), (5), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN1177235) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Peptide
Clone N46-467
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background The clone N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50
Molecular Weight ~28 kDa
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Application Notes IHC(F): Antigen retrieval required
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN1177235) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-BCL2 Antibody (ABIN1177235)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Geng, Tang, Li, Yang, Choi, Kazim, Zhang: "Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, 2011

Sarkar, Korolchuk, Renna, Imarisio, Fleming, Williams, Garcia-Arencibia, Rose, Luo, Underwood, Kroemer, OKane, Rubinsztein: "Complex inhibitory effects of nitric oxide on autophagy." in: Molecular cell, Vol. 43, Issue 1, pp. 19-32, 2011

Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, 2001

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, 1998