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anti-Mouse (Murine) BCL2 antibody for Enzyme Immunoassay

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
N-Term
165
73
63
59
41
33
25
25
24
18
15
15
15
14
13
13
13
11
8
7
6
5
5
5
5
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
988
250
210
54
27
22
18
18
5
4
4
3
2
2
2
2
Host
Goat
539
511
18
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
46
42
37
24
23
21
15
14
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
781
368
359
307
305
289
226
185
169
81
40
13
12
12
12
11
9
4
3
1
1
1
1
1
1
1
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Catalog No. ABIN374455
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN499427 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN371613 EIA WB Mouse IgG1 AA 61-76 Log in to see 10C2 12
1 ABIN950625 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
1 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
1 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012

Top referenced anti-BCL2 antibody for Enzyme Immunoassay

Similar anti-BCL2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies 6 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Western Blotting (WB) 722 Antibodies 165 Antibodies 133 Antibodies 27 Antibodies
Neutralization (Neut) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 210 Antibodies 34 Antibodies 28 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 351 Antibodies 29 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(165), (73), (63), (59), (41), (33), (25), (25), (24), (18), (15), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(988), (250), (210), (54), (27), (22), (18), (18), (5), (4), (4), (3), (2), (2), (2), (2)
Host Goat
(539), (511), (18), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (46), (42), (37), (24), (23), (21), (15), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(781), (368), (359), (307), (305), (289), (226), (185), (169), (81), (40), (13), (12), (12), (12), (11), (9), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN374455) Images
Sequence AGRTGYDNRE IVMKYC
Specificity This antibody is expected to recognise both reported isoforms (NP_000624, NP_000648) of BCL2.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the N Terminus of the protein sequence according to NP_000624.2, NP_000648.2. Genename: BCL2

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN374455) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596, 9606
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN374455) Images back to top
Application Notes Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN374455) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BCL2 Antibody (ABIN374455)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Yu, Xue, Zhou, Li, Zhou: "Tanshinone IIB, a primary active constituent from Salvia miltiorrhiza, exerts neuroprotective effect via inhibition of neuronal apoptosis in vitro." in: Phytotherapy research : PTR, Vol. 22, Issue 6, pp. 846-50, 2008

Yu, Xue, Zhou, Li, Du, Liang, Zhou: "In vitro and in vivo neuroprotective effect and mechanisms of glabridin, a major active isoflavan from Glycyrrhiza glabra (licorice)." in: Life sciences, Vol. 82, Issue 1-2, pp. 68-78, 2007

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN374455) back to top
Supplier Images
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN374455) anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody