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anti-Mouse (Murine) BCL2 antibody for Fluorescence Microscopy

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Reactivity
Mouse (Murine)
954
219
166
40
23
15
7
4
3
3
2
2
2
1
Host
Armenian Hamster
521
508
19
5
2
2
1
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
764
341
321
300
290
280
255
179
153
67
42
11
10
10
8
8
5
1
1
1
1
Supplier
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Catalog No. ABIN967441
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Top referenced anti-BCL2 antibody for Fluorescence Microscopy

Similar anti-BCL2 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 56 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 33 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 139 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Reactivity Mouse (Murine)
(954), (219), (166), (40), (23), (15), (7), (4), (3), (3), (2), (2), (2), (1)
Host Armenian Hamster
(521), (508), (19), (5), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(764), (341), (321), (300), (290), (280), (255), (179), (153), (67), (42), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN967441) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Mouse Bcl-2
Clone 3F11
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN967441) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contain t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival rather than by accelerating the rate of cell proliferation. Mouse Bcl-2 migrates at a reduced molecular weight of ~26 kD. This antibody recognizes a 26 kD band representing the mouse p26-Bcl-2 protein. Additional minor bands at 27-31 kD and 18-21 kD may be visualized. The 27-31 kD upper band may represent a larger isoform, whereas the 18-21 kD lower band may be an internal translation or proteolytic product. 3F11 does not cross-react with human Bcl-2. For detection of human Bcl-2, refer to clone 6C8 (ABIN967311), or clone 4D7.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN967441) Images back to top
Application Notes Reported ranges for usage in western blot and IHC on frozen sections, is around 5 µg/ml. The antibody should be titrated for each application. Thymus and M1 mouse myeloma cells (ATCC TIB-192) are suggested as positive controls. For immunofluroescent staining and flow cytometry, the directly conjugated formats of this clone are recommended (Cat No. 554221 and 556537).
Comment

Related Products: ABIN967311

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN967441) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-BCL2 Antibody (ABIN967441)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Miyashita, Krajewski, Krajewska, Wang, Lin, Liebermann, Hoffman, Reed: "Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo." in: Oncogene, Vol. 9, Issue 6, pp. 1799-805, 1994

Novack, Korsmeyer: "Bcl-2 protein expression during murine development." in: The American journal of pathology, Vol. 145, Issue 1, pp. 61-73, 1994

Veis, Sentman, Bach, Korsmeyer: "Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2546-54, 1993

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993

Veis, Sorenson, Shutter, Korsmeyer: "Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair." in: Cell, Vol. 75, Issue 2, pp. 229-40, 1993

Hockenbery, Nuñez, Milliman, Schreiber, Korsmeyer: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991

Williams: "Programmed cell death: apoptosis and oncogenesis." in: Cell, Vol. 65, Issue 7, pp. 1097-8, 1991

Reed, Tsujimoto, Alpers, Croce, Nowell: "Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation." in: Science (New York, N.Y.), Vol. 236, Issue 4806, pp. 1295-9, 1987

Tsujimoto, Cossman, Jaffe, Croce: "Involvement of the bcl-2 gene in human follicular lymphoma." in: Science (New York, N.Y.), Vol. 228, Issue 4706, pp. 1440-3, 1985

Background publications

Krajewski, Tanaka, Takayama, Schibler, Fenton, Reed: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, 1993

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967441) Profile of M1 mouse myeloma cells analyzed on a FACScan™ (BDIS, San Jose, CA). Cells ...
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967441) Western blot analysis of Bcl-2 expression using 3F11 and 6C8 monoclonal antibodies in...
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967441) anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (Image 3)
Flow Cytometry (FACS) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967441) anti-B-Cell CLL/lymphoma 2 (BCL2) antibody (Image 4)