anti-Rat (Rattus) GJA1 antibody for Immunohistochemistry (Frozen Sections)

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
AA 351-367, C-Term
68
21
20
18
15
13
10
8
8
7
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
281
191
174
53
22
22
21
17
16
16
10
10
7
7
6
5
5
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
261
43
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
242
119
102
81
45
29
19
14
13
8
7
4
2
2
2
Options
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Catalog No. ABIN5518645
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2621330 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367 Log in to see Polyclonal
1 ABIN199018 ICC IHC (fro) ELISA WB Mouse IgG1 AA 252-270 Log in to see
1 ABIN220025 IHC (fro) WB Mouse AA 131-142 Log in to see
1 ABIN3031019 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367 Log in to see Polyclonal

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope AA 351-367, C-Term
(68), (21), (20), (18), (15), (13), (10), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(281), (191), (174), (53), (22), (22), (21), (17), (16), (16), (10), (10), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(261), (43), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(242), (119), (102), (81), (45), (29), (19), (14), (13), (8), (7), (4), (2), (2), (2)
Pubmed 3 references available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN5518645) Images
Purpose Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence HELQPLAIVD QRPSSRA
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: gap junction protein, alpha 1, 43 kDa
Protein Name: Gap junction alpha-1 protein
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.
Isotype IgG

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 antibody (ABIN5518645) Images back to top
Antigen
Alternative Name GJA1 (GJA1 Antibody Abstract)
Background Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Synonyms: Connexin 43 antibody|Connexin-43 antibody|Connexin43 antibody|Cx 43 antibody|CX43 antibody|CXA1_HUMAN antibody|DFNB38 antibody|Gap junction 43 kDa heart protein antibody|Gap junction alpha 1 protein antibody|Gap junction alpha-1 protein antibody|Gap junction protein alpha 1 43kD antibody|Gap junction protein alpha 1 43 kDa(connexin 43) antibody|Gap junction protein alpha 1 43 kDa antibody|Gap junction protein alpha like antibody|GJA 1 antibody|Gja1 antibody|GJAL antibody|HGNC:4282 antibody|HGNC:8112 antibody|Oculodentodigital dysplasia antibody|ODD antibody|ODDD antibody|ODOD antibody|SDTY 3 antibody|SDTY3 antibody|Syndactyly type III antibody
UniProt P17302
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 antibody (ABIN5518645) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 antibody (ABIN5518645) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GJA1 antibody (ABIN5518645)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Product cited in:

Zhang, Gao, Dong, Wang, Yao, Zhang, Hu, Xu, Zuo, Wang, Zhou, Zhao, Peng: "The compound Chinese medicine "Kang Fu Ling" protects against high power microwave-induced myocardial injury." in: PLoS ONE, Vol. 9, Issue 7, pp. e101532, 2014

Li, Tang, Liang, Li, Wang, Song, Zheng, Xi, Zhang, Hescheler, Zhu: "Coculture of embryonic ventricular myocytes and mouse embryonic stem cell enhance intercellular signaling by upregulation of connexin43." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 1, pp. 53-63, 2013

Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, 2010