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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||48 Antibodies||73 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||28 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||14 Antibodies|
|Immunohistochemistry (IHC)||59 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies|
|Western Blotting (WB)||165 Antibodies|
|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Epitope||AA 351-367, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein(GJA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: gap junction protein, alpha 1, 43 kDa
Protein Name: Gap junction alpha-1 protein
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling Images back to top
|Alternative Name||GJA1 (GJA1 Antibody Abstract)|
Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Synonyms: Connexin 43 antibody|Connexin-43 antibody|Connexin43 antibody|Cx 43 antibody|CX43 antibody|CXA1_HUMAN antibody|DFNB38 antibody|Gap junction 43 kDa heart protein antibody|Gap junction alpha 1 protein antibody|Gap junction alpha-1 protein antibody|Gap junction protein alpha 1 43kD antibody|Gap junction protein alpha 1 43 kDa(connexin 43) antibody|Gap junction protein alpha 1 43 kDa antibody|Gap junction protein alpha like antibody|GJA 1 antibody|Gja1 antibody|GJAL antibody|HGNC:4282 antibody|HGNC:8112 antibody|Oculodentodigital dysplasia antibody|ODD antibody|ODDD antibody|ODOD antibody|SDTY 3 antibody|SDTY3 antibody|Syndactyly type III antibody
|Pathways||MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Sodium azide, Thimerosal (Merthiolate)|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.