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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||143 Antibodies||161 Antibodies||184 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||9 Antibodies||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||75 Antibodies||45 Antibodies||46 Antibodies|
|Immunohistochemistry (IHC)||106 Antibodies||59 Antibodies|
|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Connexin 43 protein when phosphorylated at Ser367.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling Images back to top
|Alternative Name||GJA1 / Cx43 (GJA1 Antibody Abstract)|
|Background||The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein|
|Molecular Weight||approx. 43 kDa|
|Pathways||MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling back to top