You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Chicken MAP2K1 antibody for Intracellular Staining

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
605
322
279
59
38
24
15
13
13
6
4
4
4
2
2
Host
Mouse
493
122
2
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is conjugated to PE
14
14
14
10
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Intracellular Staining (ICS)
453
208
157
116
95
66
23
22
14
10
8
6
6
2
2
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1176908
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1176909 ICS Alexa Fluor 647 Mouse IgG2a Log in to see 25-MEK1 3

Top referenced anti-MAP2K1 antibody for Intracellular Staining

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken
Western Blotting (WB) 441 Antibodies 232 Antibodies 207 Antibodies 56 Antibodies 12 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies 9 Antibodies 9 Antibodies 3 Antibodies
Immunoprecipitation (IP) 14 Antibodies 10 Antibodies 2 Antibodies 2 Antibodies 8 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(605), (322), (279), (59), (38), (24), (15), (13), (13), (6), (4), (4), (4), (2), (2)
Host Mouse
(493), (122), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is conjugated to PE
(14), (14), (14), (10), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Intracellular Staining (ICS)
(453), (208), (157), (116), (95), (66), (23), (22), (14), (10), (8), (6), (6), (2), (2), (2), (2), (2)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN1176908) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human MEK1 Recombinant Protein
Clone 25-MEK1
Isotype IgG2a

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN1176908) Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background MEK1 (MapK/ERK Kinase 1) is a 45-kDa member of the MEK family of dual specificity kinases. MEK is activated by a variety of cellular serine/threonine kinases including c-Raf, A-Raf, c-mos, and MEK Kinase-1. Activated MEK phosphorylates MAP kinase (ERK) at threonine and tyrosine residues. This results in activation of ERK and its signaling pathway. MEK is highly specific for ERK and various MEKs preferentially phosphorylate individual ERK isoforms. MEK1 only activates ERK1 and ERK2. This specificity may result from variations in ERK regions that are known as the phosphorylation lip and kinase backbone. MEK's localization is cytoplasmic, but mitogenic stimulation induces a mass translocation to the nucleus. Mechanisms behind this nuclear translocation remain unknown. However, MEK contains an N-terminal nuclear export signal (NES) that mediates its rapid exodus from the nucleus and restores its unstimulated cellular distribution. The 25/MEK1 monoclonal antibody recognizes MEK1, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: MAPK/ERK kinase 1, EC 2.7.12.2, kinase MEK1, MAPKK1, PRKMK1
Research Area Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN1176908) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN1176908) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-MAP2K1 Antibody (ABIN1176908)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Product cited in:

Gu, Fujibayashi, Yamada, Sekiguchi: "Laminin-10/11 and fibronectin differentially prevent apoptosis induced by serum removal via phosphatidylinositol 3-kinase/Akt- and MEK1/ERK-dependent pathways." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19922-8, 2002

Aplin, Stewart, Assoian, Juliano: "Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1." in: The Journal of cell biology, Vol. 153, Issue 2, pp. 273-82, 2001

Robinson, Cheng, Khokhlatchev, Ebert, Ahn, Guan, Stein, Goldsmith, Cobb: "Contributions of the mitogen-activated protein (MAP) kinase backbone and phosphorylation loop to MEK specificity." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29734-9, 1997