anti-Rat (Rattus) MAP2K1 antibody for Immunoprecipitation

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
645
427
374
108
80
45
30
25
22
21
10
8
5
5
4
4
4
2
1
1
Host
Mouse
516
128
5
4
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is un-conjugated
16
15
15
11
9
8
8
8
6
6
6
6
6
6
6
6
6
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
506
203
162
133
123
78
27
22
19
10
9
7
6
6
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2853620
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967762 BI IP IHC WB Mouse IgG2a Log in to see 25-MEK1 5
1 ABIN967763 BI IP IHC WB Mouse IgG2a Log in to see 25-MEK1 5
1 ABIN4231966 IP WB Mouse IgG2a Log in to see 4A5
1 ABIN1493436 ICC IHC (p) IP WB Sepharose Rabbit AA 353-367 Log in to see Polyclonal
1 ABIN1493435 ICC IHC (p) IP WB Magnetic Particles Rabbit AA 353-367 Log in to see Polyclonal
1 ABIN1959461 IF IP IHC (p) PLA Rabbit IgG pThr286 Log in to see Polyclonal
1 ABIN1959462 IF IP IHC (p) Goat IgG pThr286 Log in to see Polyclonal
1 ABIN1584755 IP IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN1818528 IP WB Mouse IgG2a, kappa Log in to see 4A5
1 ABIN2314664 IP IHC ELISA WB Rabbit IgG AA 1-12, N-Term Log in to see Polyclonal
1 ABIN2586958 IP WB Mouse IgG2a Log in to see 4A5
1 ABIN1690250 IP Magnetic Particles Rabbit Log in to see Polyclonal
1 ABIN1690597 IP Sepharose Rabbit Log in to see Polyclonal
1 ABIN5558363 ELISA IHC IP WB Biotin Chicken Log in to see Polyclonal
1 ABIN5558364 ELISA IHC IP WB FITC Chicken Log in to see Polyclonal
1 ABIN5558362 ELISA IHC IP WB Chicken Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(645), (427), (374), (108), (80), (45), (30), (25), (22), (21), (10), (8), (5), (5), (4), (4), (4), (2), (1), (1)
Host Mouse
(516), (128), (5), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is un-conjugated
(16), (15), (15), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(506), (203), (162), (133), (123), (78), (27), (22), (19), (10), (9), (7), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN2853620) Images
Specificity This antibody reacts with MAPKK (MEK1).
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat, xenopus
Purification Protein A agarose
Immunogen Recombinant Xenopus MAPKK
Clone 4A5
Isotype IgG2a

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 antibody (ABIN2853620) Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background The MAP kinases mediate a wide range of cellular functions via a variety of signal transduction pathways in eukaryotic cells. The most studied mammalian MAP kinase pathway is the Ras-Raf-MEK-ERK pathway. The binding of GTP to Ras protein initiates a phosphorylation cascade through Raf-1 and MEK1/2 (MAPK kinase), which results in stimulation of the MAP kinases, ERK1/2. Upon stimulation, ERKs are known to phosphorylate a variety of cytosolic substrates and are also translocated into the nucleus where they initiate the transcription of immediate early genes. The Ras-Raf-MEK-ERK pathway is stimulated by various growth factors and extracellular stimuli and plays important roles in cell survival, differentiation and proliferation. The observation that MEK1/2 inhibitors potentiate the antitumor activity of various cytotoxic agents, including ara-C, cisplatin and paclitaxel, suggests a possible role for MEK1/2 inhibitors in the treatment of human malignancies.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 antibody (ABIN2853620) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 antibody (ABIN2853620) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-MAP2K1 antibody (ABIN2853620)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Background publications

Miura, Lavens-Phillips, MacGlashan: "Localizing a control region in the pathway to leukotriene C(4) secretion following stimulation of human basophils with anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 12, pp. 7027-37, 2001

Fukuda, Gotoh, Gotoh, Nishida: "Cytoplasmic localization of mitogen-activated protein kinase kinase directed by its NH2-terminal, leucine-rich short amino acid sequence, which acts as a nuclear export signal." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 20024-8, 1996

Fukuda, Gotoh, Kosako, Hattori, Nishida: "Analysis of the Ras p21/mitogen-activated protein kinase signaling in vitro and in Xenopus oocytes." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33097-101, 1995

Riesmeier, Willmitzer, Frommer: "Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning." in: The EMBO journal, Vol. 13, Issue 1, pp. 1-7, 1994

Otsu, Terada, Okayama: "Isolation of two members of the rat MAP kinase kinase gene family." in: FEBS letters, Vol. 320, Issue 3, pp. 246-50, 1993

Kosako, Nishida, Gotoh: "cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates." in: The EMBO journal, Vol. 12, Issue 2, pp. 787-94, 1993

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN2853620) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody (ABIN2853620) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody (ABIN2853620) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody (Image 2)