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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Xenopus laevis|
|Western Blotting (WB)||415 Antibodies||220 Antibodies||194 Antibodies||14 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Luminex Assay (LMNX)||10 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies||9 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies||10 Antibodies||7 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||88 Antibodies||44 Antibodies||38 Antibodies||2 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images
|Specificity||This antibody reacts with MAPKK (MEK1).|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat, xenopus|
|Purification||Protein A agarose|
|Immunogen||Recombinant Xenopus MAPKK|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||The MAP kinases mediate a wide range of cellular functions via a variety of signal transduction pathways in eukaryotic cells. The most studied mammalian MAP kinase pathway is the Ras-Raf-MEK-ERK pathway. The binding of GTP to Ras protein initiates a phosphorylation cascade through Raf-1 and MEK1/2 (MAPK kinase), which results in stimulation of the MAP kinases, ERK1/2. Upon stimulation, ERKs are known to phosphorylate a variety of cytosolic substrates and are also translocated into the nucleus where they initiate the transcription of immediate early genes. The Ras-Raf-MEK-ERK pathway is stimulated by various growth factors and extracellular stimuli and plays important roles in cell survival, differentiation and proliferation. The observation that MEK1/2 inhibitors potentiate the antitumor activity of various cytotoxic agents, including ara-C, cisplatin and paclitaxel, suggests a possible role for MEK1/2 inhibitors in the treatment of human malignancies.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
|Buffer||PBS containing 50 % glycerol|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-MAP2K1 Antibody (ABIN2853620)Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Miura, Lavens-Phillips, MacGlashan: "Localizing a control region in the pathway to leukotriene C(4) secretion following stimulation of human basophils with anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 12, pp. 7027-37, 2001 (PubMed).
Fukuda, Gotoh, Gotoh et al.: "Cytoplasmic localization of mitogen-activated protein kinase kinase directed by its NH2-terminal, leucine-rich short amino acid sequence, which acts as a nuclear export signal." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 20024-8, 1996 (PubMed).
Otsu, Terada, Okayama: "Isolation of two members of the rat MAP kinase kinase gene family." in: FEBS letters, Vol. 320, Issue 3, pp. 246-50, 1993 (PubMed).
Kosako, Nishida, Gotoh: "cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates." in: The EMBO journal, Vol. 12, Issue 2, pp. 787-94, 1993 (PubMed).
Riesmeier, Willmitzer, Frommer: "Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning." in: The EMBO journal, Vol. 13, Issue 1, pp. 1-7, 1994 (PubMed).
Fukuda, Gotoh, Kosako et al.: "Analysis of the Ras p21/mitogen-activated protein kinase signaling in vitro and in Xenopus oocytes." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33097-101, 1995 (PubMed).
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) back to top