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anti-Rat (Rattus) MAP2K1 antibody for Western Blotting

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Reactivity
Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
605
322
279
60
38
24
14
14
13
6
4
4
4
2
2
Host
Mouse
493
122
2
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
452
208
157
116
95
66
23
21
14
10
9
6
6
2
2
2
2
2
Supplier
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Catalog No. ABIN2853620
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1347588 ABIN967762 BI IP IHC WB Mouse IgG2a Log in to see 25-MEK1 5
0.1347588 ABIN967763 BI IP IHC WB Mouse IgG2a Log in to see 25-MEK1 5
0.1347588 ABIN152969 WB Rabbit Thr386 Log in to see Polyclonal 2
0.1347588 ABIN126835 EIA WB Mouse IgG1 Log in to see 10B1
0.1347588 ABIN126837 EIA WB Mouse IgG2a Log in to see 9G3
0.1347588 ABIN1531442 ELISA WB Rabbit IgG pSer298 Log in to see Polyclonal
0.1347588 ABIN1531443 IHC ELISA WB Rabbit IgG pThr286 Log in to see Polyclonal
0.1347588 ABIN1531904 ELISA WB Rabbit IgG pThr291 Log in to see Polyclonal
0.1347588 ABIN498721 IHC (p) WB Rabbit pSer222, pSer226 Log in to see Polyclonal
0.1347588 ABIN498700 IF IHC (p) WB Rabbit pThr292 Log in to see Polyclonal
0.1347588 ABIN498720 IHC (p) WB Rabbit pSer218, pSer222 Log in to see Polyclonal
0.1347588 ABIN1532459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.1347588 ABIN1532460 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.1347588 ABIN1532935 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.1347588 ABIN3044391 ICC IHC (p) WB Rabbit IgG AA 353-367, C-Term Log in to see Polyclonal
0.1347588 ABIN498600 IHC (p) WB Rabbit Log in to see Polyclonal
0.1347588 ABIN498599 WB Rabbit Log in to see Polyclonal
0.1347588 ABIN498654 IHC (p) WB Rabbit Log in to see Polyclonal
0.1347588 ABIN615073 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.1347588 ABIN1870339 IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal

Top referenced anti-MAP2K1 antibody for Western Blotting

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Xenopus laevis
Western Blotting (WB) 441 Antibodies 232 Antibodies 207 Antibodies 15 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Intracellular Staining (ICS) 9 Antibodies 9 Antibodies 3 Antibodies
Immunoprecipitation (IP) 14 Antibodies 10 Antibodies 8 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies 49 Antibodies 44 Antibodies 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(605), (322), (279), (60), (38), (24), (14), (14), (13), (6), (4), (4), (4), (2), (2)
Host Mouse
(493), (122), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(452), (208), (157), (116), (95), (66), (23), (21), (14), (10), (9), (6), (6), (2), (2), (2), (2), (2)
Pubmed 6 references available
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images
Specificity This antibody reacts with MAPKK (MEK1).
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat, xenopus
Purification Protein A agarose
Immunogen Recombinant Xenopus MAPKK
Clone 4A5
Isotype IgG2a

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background The MAP kinases mediate a wide range of cellular functions via a variety of signal transduction pathways in eukaryotic cells. The most studied mammalian MAP kinase pathway is the Ras-Raf-MEK-ERK pathway. The binding of GTP to Ras protein initiates a phosphorylation cascade through Raf-1 and MEK1/2 (MAPK kinase), which results in stimulation of the MAP kinases, ERK1/2. Upon stimulation, ERKs are known to phosphorylate a variety of cytosolic substrates and are also translocated into the nucleus where they initiate the transcription of immediate early genes. The Ras-Raf-MEK-ERK pathway is stimulated by various growth factors and extracellular stimuli and plays important roles in cell survival, differentiation and proliferation. The observation that MEK1/2 inhibitors potentiate the antitumor activity of various cytotoxic agents, including ara-C, cisplatin and paclitaxel, suggests a possible role for MEK1/2 inhibitors in the treatment of human malignancies.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN2853620) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-MAP2K1 Antibody (ABIN2853620)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Background publications

Miura, Lavens-Phillips, MacGlashan: "Localizing a control region in the pathway to leukotriene C(4) secretion following stimulation of human basophils with anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 12, pp. 7027-37, 2001

Fukuda, Gotoh, Gotoh, Nishida: "Cytoplasmic localization of mitogen-activated protein kinase kinase directed by its NH2-terminal, leucine-rich short amino acid sequence, which acts as a nuclear export signal." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 20024-8, 1996

Fukuda, Gotoh, Kosako, Hattori, Nishida: "Analysis of the Ras p21/mitogen-activated protein kinase signaling in vitro and in Xenopus oocytes." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33097-101, 1995

Riesmeier, Willmitzer, Frommer: "Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning." in: The EMBO journal, Vol. 13, Issue 1, pp. 1-7, 1994

Otsu, Terada, Okayama: "Isolation of two members of the rat MAP kinase kinase gene family." in: FEBS letters, Vol. 320, Issue 3, pp. 246-50, 1993

Kosako, Nishida, Gotoh: "cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates." in: The EMBO journal, Vol. 12, Issue 2, pp. 787-94, 1993

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN2853620) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (ABIN2853620) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (ABIN2853620) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody (Image 2)