anti-Human MAP2K4 antibody for Western Blotting

Recommended MAP2K4 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
  • jnkk
  • mek4
  • mkk4
  • sek1
  • jnkk1
  • serk1
  • xmek2
  • mapkk4
  • prkmk4
  • 142758_at
  • CG9738
  • CT27508
  • D-MKK4
  • DMKK4
  • Dmel\\CG9738
  • JNKK2
  • MAPKK4
  • MKK4
  • Mpk4
  • SEK1/MKK4
  • dMKK4
  • dMkk4
  • hep
  • CT38386
  • DmelCG12798
  • Sek1
  • 2
  • JNKK
  • Jnkk2
  • Mapkk7
  • Mek7
  • Mkk7
  • JNKK1
  • MEK4
  • PRKMK4
  • SAPKK-1
  • SAPKK1
  • SEK1
  • SERK1
  • SKK1
  • Serk1
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4
  • ATMEK4
  • ATMKK4
  • F19C24.26
  • F19C24_26
  • MAP KINASE KINASE 4
  • mitogen-activated protein kinase kinase 4
  • MKK4A-l
  • map2k4
  • zgc:136500
  • mitogen-activated protein kinase kinase 4
  • MAP kinase kinase 4
  • CG12798
  • mitogen activated protein kinase kinase 4
  • mitogen activated protein kinase kinase 7
  • mitogen-activated protein kinase kinase 4a
  • map2k4
  • Mkk4
  • CG12798
  • Map2k4
  • Map2k7
  • MAP2K4
  • MKK4
  • map2k4a
Reactivity
Dog (Canine), Human, Mouse (Murine)
325
178
139
15
8
8
8
4
3
3
2
1
1
1
1
Host
Rabbit
294
42
Clonality
Polyclonal
Conjugate
This MAP2K4 antibody is un-conjugated
12
12
11
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
230
156
122
61
57
22
15
14
14
10
6
4
3
1
Options
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Catalog No. ABIN223508
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2214506 ABIN1531968 IHC ELISA WB Rabbit IgG AA 227-276, pThr261 Log in to see Polyclonal 2
3.2214506 ABIN1533737 ELISA WB Rabbit IgG AA 316-365 Log in to see Polyclonal 2
3.2214506 ABIN2706553 IC IF IHC WB Rabbit pSer261 Log in to see Polyclonal
3.2214506 ABIN2706554 WB Rabbit Center Log in to see Polyclonal
3.2214506 ABIN2706552 IC IF IP IHC WB Rabbit pSer80 Log in to see Polyclonal
3.2214506 ABIN1533001 ELISA WB Rabbit IgG AA 46-95 Log in to see Polyclonal
3.2214506 ABIN1531967 IHC ELISA WB Rabbit IgG AA 46-95, pSer80 Log in to see Polyclonal
3.2214506 ABIN658838 IHC (p) WB Rabbit Ig AA 259-287, C-Term Log in to see Polyclonal 2
3.2214506 ABIN3182071 ELISA IHC WB Rabbit IgG pThr261 Log in to see Polyclonal
3.2214506 ABIN3185529 ELISA WB Rabbit IgG Tyr104 Log in to see Polyclonal
3.2214506 ABIN3182070 ELISA IHC WB Rabbit IgG pSer80 Log in to see Polyclonal
3.2214506 ABIN969268 FACS IHC ELISA WB Mouse IgG1 Log in to see 5H4 2
3.2214506 ABIN407820 WB Rabbit IgG Log in to see Polyclonal
3.2214506 ABIN3042559 WB Rabbit IgG AA 360-378, C-Term Log in to see Polyclonal
3.2214506 ABIN4334803 IHC IHC (p) WB Rabbit pSer80 Log in to see Polyclonal 1
3.2214506 ABIN3186893 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.2214506 ABIN1870354 IF IHC WB Rabbit IgG pSer80 Log in to see Polyclonal
3.2214506 ABIN2452439 IF IP IHC WB Mouse IgG1 Log in to see 2D5
3.2214506 ABIN2801269 WB Rabbit C-Term Log in to see Polyclonal
3.2214506 ABIN498316 IF IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(325), (178), (139), (15), (8), (8), (8), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(294), (42)
Clonality
Polyclonal
Conjugate This MAP2K4 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(230), (156), (122), (61), (57), (22), (15), (14), (14), (10), (6), (4), (3), (1)
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Product Details anti-MAP2K4 Antibody

Target Details MAP2K4 Application Details Handling Images
Purpose Affinity purified polyclonal antibody to detect SEK1 (MKK4/JKK1) in human, mouse and dog samples.
Blocking Peptide Blocking peptide for this product available: ABIN412828
Specificity The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins.
Characteristics Affinity purified polyclonal antibody to detect SEK1 (MKK4/JKK1) in human, mouse and dog samples.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 347 of mouse SEK1
Isotype IgG

Target Details MAP2K4

Product Details anti-MAP2K4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SEK1/MKK4/JKK1 (MAP2K4 Antibody Abstract)
Background SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs throμgh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
Synonyms: NKK , JNKK1 , MAP kinase kinase 4 , MAP2K4 , MAPK/ERK kinase 4 , MAPKK4 , MEK4 , monkeyK4 , PRKMK4 , SAPK/ERK kinase 1 , SEK1 ,SERK1
Gene ID 26398
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling Images back to top
Application Notes Western blotting (0.5-4 μg/mL). However, the optimal concentrations should be determined individually. The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins.
Comment

Suggested positive control:
Western Blot: Jurkat cell lysate

Restrictions For Research Use only

Handling

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

Images

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) antibody (ABIN223508) Western blot analysis of SEK1 expression with Jurkat cell lysate (Lane 1, 2).