|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||38 Antibodies||114 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||4 Antibodies|
|Immunochromatography (IC)||3 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||35 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||15 Antibodies|
|Immunohistochemistry (IHC)||79 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||37 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||113 Antibodies|
|Dot Blot (DB)||14 Antibodies|
|Flow Cytometry (FACS)||12 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAP2K4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-MAP2K4 AntibodyTarget Details MAP2K4 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of SEK1/MKK4 protein. (region surrounding Glu74)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details MAP2K4Product Details anti-MAP2K4 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K4 (MAP2K4 Antibody Abstract)|
|Background||The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.Synonyms: Dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, MAP Kinase Kinase 4, MAPKK4, MEK-4, MKK4, PRKMK4, SAPK/ERK kinase 1, SERK1, c-Jun N-terminal kinase kinase 1|
|Molecular Weight||approx. 44 kDa|
|Pathways||MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling back to top