anti-Mouse (Murine) MAPK11 antibody for Western Blotting

Recommended MAPK11 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 11 (MAPK11) Antibodies
  • ATMPK11
  • F22L4.10
  • F22L4_10
  • MAP kinase 11
  • zgc:86905
  • P38B
  • P38BETA2
  • PRKM11
  • SAPK2
  • SAPK2B
  • p38-2
  • p38Beta
  • P38b
  • Prkm11
  • Sapk2
  • Sapk2b
  • p38beta
  • p38beta2
  • p38b
  • sapk2
  • prkm11
  • sapk2b
  • mitogen-activated protein kinase 11
  • mapk11
  • ARALYDRAFT_887292
  • MPK11
  • MAPK11
  • Mapk11
  • PTRG_02565
Epitope
N-Term
17
16
16
15
13
12
9
8
7
7
6
6
5
4
4
3
2
2
1
1
Reactivity
Mouse (Murine)
138
83
48
2
2
1
Host
Rabbit
118
23
3
Clonality
Polyclonal
Conjugate
This MAPK11 antibody is un-conjugated
8
8
6
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
108
59
46
26
13
8
6
3
2
2
1
1
1
Options
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Available images

Catalog No. ABIN2798348
$ 454.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1581392 ABIN359418 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 5
3.1581392 ABIN2786701 WB Rabbit N-Term Log in to see Polyclonal
3.1581392 ABIN391730 WB Rabbit Ig AA 334-359, C-Term Log in to see Polyclonal 5
3.1581392 ABIN657841 IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal
3.1581392 ABIN391328 IHC (p) WB Rabbit Ig AA 334-366, Center Log in to see Polyclonal
3.1581392 ABIN2737137 IHC WB Rabbit IgG Log in to see Polyclonal
3.1581392 ABIN391731 WB Rabbit Ig AA 133-161, Center Log in to see Polyclonal
3.1581392 ABIN3198308 IHC WB Rabbit Log in to see Polyclonal
3.1581392 ABIN4342700 WB Rabbit Log in to see Polyclonal
3.1581392 ABIN3031829 ELISA WB Rabbit Ig Fraction AA 334-359 Log in to see Polyclonal
3.1581392 ABIN3031828 ELISA WB Rabbit Ig Fraction AA 133-161 Log in to see Polyclonal
3.1581392 ABIN2426617 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN208500 WB Rabbit IgG AA 39-59 Log in to see Polyclonal
1 ABIN754393 IF (p) IHC (p) WB Rabbit IgG AA 20-70 Log in to see Polyclonal
1 ABIN710576 IF (p) IHC (p) WB Rabbit IgG pThr180, pTyr182 Log in to see Polyclonal
1 ABIN754402 IHC (p) WB HRP Rabbit IgG AA 20-70 Log in to see Polyclonal
1 ABIN1911888 IHC ELISA WB APC Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1911887 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1911891 IHC ELISA WB PE Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1911889 IHC ELISA WB Biotin Rabbit IgG Internal Region Log in to see Polyclonal

Similar anti-MAPK11 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 23 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 11 (MAPK11) Antibodies
Epitope N-Term
(17), (16), (16), (15), (13), (12), (9), (8), (7), (7), (6), (6), (5), (4), (4), (3), (2), (2), (1), (1)
Reactivity Mouse (Murine)
(138), (83), (48), (2), (2), (1)
Host Rabbit
(118), (23), (3)
Clonality
Polyclonal
Conjugate This MAPK11 antibody is un-conjugated
(8), (8), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(108), (59), (46), (26), (13), (8), (6), (3), (2), (2), (1), (1), (1)
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Product Details anti-MAPK11 Antibody

Target Details MAPK11 Application Details Handling Images
Isotype Ig Fraction

Target Details MAPK11

Product Details anti-MAPK11 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mapk11 (MAPK11 Antibody Abstract)
Molecular Weight 41397 Da
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Response to Water Deprivation, Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Hepatitis C, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-MAPK11 Antibody Target Details MAPK11 Handling Images back to top
Application Notes Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Comment

Rabbit Anti-Mouse Mouse Mapk11 (N-term) Antibody, 400 μl

Restrictions For Research Use only

Handling

Product Details anti-MAPK11 Antibody Target Details MAPK11 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Storage 4 °C,-20 °C
'Independent Validation' Badge
Antigen MAPK11
Lot Number RAY8110713
Method validated Western Blotting
Positive Control primary mouse microglia
Negative Control siRNA knock-down of MAPK11 in primary mouse microglia
Notes Passed. ABIN2798348 specifically recognizes Mapk11 in primary mouse microglia lysates.
Primary Antibody ABIN2798348
Secondary Antibody anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
Protocol
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse MAPK11 Silencer Select siRNA (ThermoFisher Scientific, Sample ID ASO10943, ASO1098J and ASO109CX, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary rabbit anti-MAPK11 antibody (antibodies-online, ABIN2798348, lot RAY8110713) diluted 1:500 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Experimental Notes The MAPK11 antibody ABIN2798348 reveals a protein of the expected molecular weight of murine Mapk11 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (N-Term) antibody (ABIN2798348) Primary mouse microglia were transfected with Mapk11 siRNA and Mapk11 was revealed us...