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anti-Human MAPK14 antibody for Western Blotting

Recommended MAPK14 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
  • 186F5S
  • anon-sts23
  • AP22.98
  • AP22_98
  • ATMPK14
  • BG:DS00797.3
  • CG7393
  • CRK1
  • Crk1
  • CSBP
  • csbp
  • Csbp1
  • CSBP1
  • Csbp2
  • CSBP2
  • CSPB1
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dm p38b
  • Dp38
  • dp38b
  • Dp38b
  • ESTS:186F5S
  • Exip
  • EXIP
  • Hog
  • mapk14a
  • mitogen-activated protein kinase 14
  • Mpk34C
  • Mxi2
  • mxi2
  • p38
  • p38 beta
  • p38 MAPK
  • p38-alpha
  • p38a
  • p38alpha
  • p38ALPHA
  • p38B
  • p38beta
  • p38Hog
  • p38Kb
  • p38MAPK
  • Prkm14
  • PRKM14
  • Prkm15
  • PRKM15
  • RK
  • sapk2
  • sapk2a
  • SAPK2A
  • Sapk2A
  • SAPK2a
Epitope
Tyr322
29
25
17
13
12
11
10
9
7
7
7
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
178
81
73
18
8
7
6
4
3
2
2
2
2
2
1
1
Host
Rabbit
151
30
2
1
Clonality
Polyclonal
Conjugate
This MAPK14 antibody is un-conjugated
7
7
6
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
163
77
53
28
23
10
10
8
7
6
5
2
1
Supplier
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Catalog No. ABIN359422
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12689514 ABIN391334 IHC (p) WB Rabbit Ig AA 158-192, Thr180, Tyr182 Log in to see Polyclonal 2
0.12689514 ABIN2792183 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.12689514 ABIN2792184 WB Rabbit C-Term Log in to see Polyclonal 1
0.12689514 ABIN359424 EIA WB Rabbit Ig Thr180 Log in to see Polyclonal 5
0.12689514 ABIN1724904 IHC ELISA WB Mouse IgG1 AA 299-360 Log in to see 10B11C8 2
0.12689514 ABIN1724830 ICC FACS IHC ELISA WB Mouse IgG1 AA 299-360 Log in to see 8G4D11 2
0.12689514 ABIN968769 IF WB Mouse IgG1 AA 243-355 Log in to see 27-p38alpha-SAPK2a 4
0.12689514 ABIN968770 IF WB Mouse IgG1 AA 243-355 Log in to see 27-p38alpha-SAPK2a 4
0.12689514 ABIN1882176 FACS IF WB Rabbit AA 295-324, C-Term Log in to see Polyclonal 7
0.12689514 ABIN126843 EIA IP WB Mouse IgG1 N-Term Log in to see 2F2
0.12689514 ABIN126883 EIA IF IP WB Mouse IgG1 N-Term Log in to see 13D5
0.12689514 ABIN126884 EIA WB Mouse IgG1 N-Term Log in to see 20B11
0.12689514 ABIN498749 IHC (p) WB Rabbit pTyr182 Log in to see Polyclonal
0.12689514 ABIN498575 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.12689514 ABIN498576 IHC (p) WB Rabbit Log in to see Polyclonal
0.12689514 ABIN1539222 WB Rabbit Ig AA 26-53, N-Term Log in to see Polyclonal
0.12689514 ABIN1870383 WB Rabbit IgG pThr180, pTyr182 Log in to see Polyclonal
0.12689514 ABIN1870382 IHC WB Rabbit IgG pTyr182 Log in to see Polyclonal
0.12689514 ABIN1870380 IHC WB Rabbit IgG pThr180 Log in to see Polyclonal
0.12689514 ABIN2463688 IHC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-MAPK14 antibody for Western Blotting

Similar anti-MAPK14 Antibodies

Application / Reactivity Human
Dot Blot (DB) 10 Antibodies
ELISA 78 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 161 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
Epitope Tyr322
(29), (25), (17), (13), (12), (11), (10), (9), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(178), (81), (73), (18), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(151), (30), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK14 antibody is un-conjugated
(7), (7), (6), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(163), (77), (53), (28), (23), (10), (10), (8), (7), (6), (5), (2), (1)
Pubmed 5 references available
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Product Details anti-MAPK14 Antibody

Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN359422) Images
Specificity This antibody detects MAPK14 (p38).
Purification Protein A column, followed by peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y322 of human MAPK14.
Isotype Ig

Target Details MAPK14

Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 Antibody (ABIN359422) Images back to top
Antigen
Alternative Name MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)
Background MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha
Molecular Weight 41293 Da
Gene ID 1432, 9606
UniProt Q16539
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 Antibody (ABIN359422) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 Antibody (ABIN359422) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-MAPK14 Antibody (ABIN359422)

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Background publications

Cheung, Campbell, Nebreda, Cohen: "Feedback control of the protein kinase TAK1 by SAPK2a/p38alpha." in: The EMBO journal, Vol. 22, Issue 21, pp. 5793-805, 2003

Dean, Sarsfield, Tsounakou, Saklatvala: "p38 Mitogen-activated protein kinase stabilizes mRNAs that contain cyclooxygenase-2 and tumor necrosis factor AU-rich elements by inhibiting deadenylation." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39470-6, 2003

Sun, Liu, Nguyen, Bing: "P38 MAP kinase is activated at early stages in Alzheimer's disease brain." in: Experimental neurology, Vol. 183, Issue 2, pp. 394-405, 2003

Yustein, Xia, Kahlenburg, Robinson, Templeton, Kung: "Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38." in: Oncogene, Vol. 22, Issue 40, pp. 6129-41, 2003

Frevel, Bakheet, Silva, Hissong, Khabar, Williams: "p38 Mitogen-activated protein kinase-dependent and -independent signaling of mRNA stability of AU-rich element-containing transcripts." in: Molecular and cellular biology, Vol. 23, Issue 2, pp. 425-36, 2003

Images

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN359422) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK14 antibody (Mitogen-Activated Protein Kinase 14) (Tyr322) (ABIN359422) Western blot analysis of MAPK14 Antibody (Y322) in HepG2 cell line lysates (35 µg/lan...
Western Blotting (WB) image for anti-MAPK14 antibody (Mitogen-Activated Protein Kinase 14) (Tyr322) (ABIN359422) Western blot analysis of MAPK14(arrow) using rabbit polyclonal MAPK14 Antibody (Y322)...