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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||9 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||155 Antibodies||67 Antibodies||59 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||11 Antibodies||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies||7 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||21 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK14 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAPK14 AntibodyTarget Details MAPK14 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-p38 protein only when phosphorylated at Tyr182.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details MAPK14Product Details anti-MAPK14 Antibody Application Details Handling Images back to top
|Alternative Name||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)|
|Background||MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38alpha, p38beta and p38gamma, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38alpha, p38beta and p38gamma are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, ERK6, MAPK11, MAPK12, MAPK14, MXI2, Mitogen-activated protein kinase p38, PRKM11, SAPK2, SAPK3, p38 alpha, p38 beta, p38 gamma, p38-2|
|Molecular Weight||approx. 42 kDa|
|Pathways||MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAPK14 Antibody Target Details MAPK14 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling back to top