|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Xenopus laevis|
|Western Blotting (WB)||284 Antibodies||123 Antibodies||103 Antibodies||22 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies||14 Antibodies||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||56 Antibodies||24 Antibodies||23 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||115 Antibodies||60 Antibodies||67 Antibodies||18 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies||5 Antibodies||5 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Epitope||pThr202, pTyr204 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Dot Blot (DB), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN265088) Images
|Specificity||This antibody reacts to ERK/MAPK (pThr202 / pTyr204). Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity.|
|Immunogen||Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN265088) Images back to top
|Alternative Name||MAPK3 / ERK1 (MAPK3 Antibody Abstract)|
|Background||Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN265088) Images back to top
Western Blot: 1: 1000 dilution to provide for 10 miniblots. Dot Blot: 1: 1000 dilution to provide for 10 miniblots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN265088) Images back to top
|Buffer||HEPES ( pH 7.5) solution containing 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Ship on gel ice. Store (in aliquots) at -20 °C to -70 °C.|
References for anti-MAPK3 Antibody (ABIN265088)Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994 (PubMed).
ImagesProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN265088) back to top