anti-Xenopus laevis MAPK3 antibody for Dot Blot

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • ERK1
  • fi06b09
  • wu:fi06b09
  • zERK1
  • ERK-1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • MAPK1
  • MNK1
  • Tb08.10J17.940
  • mitogen-activated protein kinase 3
  • MAP kinase-like orf; aka CEK1; one of two protein kinase genes similar to S. cerevisiae MAP kinases FUS3 (YBL016W) and KSS1 (YGR040W) involved in pheromone signal transduction
  • mitogen activated protein kinase 3
  • MPK3
  • MAPK3
  • ERK1
  • Mapk3
  • mapk3
  • Tb927.8.3550
  • Tc00.1047053509475.10
Epitope
pThr202, pTyr204
48
48
45
39
21
21
19
16
15
14
9
8
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
486
260
194
35
26
24
23
23
20
20
13
12
11
10
9
7
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
324
160
20
6
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
26
24
22
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Western Blotting (WB)
404
182
163
102
86
57
56
47
39
15
13
8
7
4
3
2
2
2
1
1
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Catalog No. ABIN265088
$ 511.50
Plus shipping costs $45.00

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope pThr202, pTyr204
(48), (48), (45), (39), (21), (21), (19), (16), (15), (14), (9), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(486), (260), (194), (35), (26), (24), (23), (23), (20), (20), (13), (12), (11), (10), (9), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(324), (160), (20), (6), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(26), (24), (22), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB), Western Blotting (WB)
(404), (182), (163), (102), (86), (57), (56), (47), (39), (15), (13), (8), (7), (4), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN265088) Images
Specificity This antibody reacts to ERK/MAPK (pThr202 / pTyr204). Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity.
Purification Affinity Purified
Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 antibody (ABIN265088) Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
NCBI Accession NP_002737
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 antibody (ABIN265088) Images back to top
Application Notes Western Blot: 1: 1000 dilution to provide for 10 miniblots. Dot Blot: 1: 1000 dilution to provide for 10 miniblots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 antibody (ABIN265088) Images back to top
Buffer HEPES ( pH 7.5) solution containing 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Ship on gel ice. Store (in aliquots) at -20 °C to -70 °C.

References for anti-MAPK3 antibody (ABIN265088)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN265088) back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr202), (pTyr204) antibody (ABIN265088) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr202), (pTyr204) antibody