anti-Mouse (Murine) MAPKAP Kinase 5 antibody for Enzyme Immunoassay

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAPK5
  • MK5
  • PRAK
  • MAPKAP-K5
  • MK-5
  • mitogen-activated protein kinase-activated protein kinase 5
  • MAP kinase-activated protein kinase 5
  • MAPKAPK5
  • mapkapk5
  • Mapkapk5
Epitope
pSer93
33
20
18
15
12
10
7
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
160
79
15
8
7
7
3
3
3
3
3
2
2
Host
Rabbit
105
47
24
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
151
87
40
22
14
13
11
4
3
1
1
1
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Catalog No. ABIN358154
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5541351 EIA IHC (p) IP WB Goat Internal Region Log in to see Polyclonal
1 ABIN5541352 EIA IHC (p) IP WB Goat AA 192-206, Internal Region Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Epitope pSer93
(33), (20), (18), (15), (12), (10), (7), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(160), (79), (15), (8), (7), (7), (3), (3), (3), (3), (3), (2), (2)
Host Rabbit
(105), (47), (24)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(151), (87), (40), (22), (14), (13), (11), (4), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 antibody (ABIN358154) Images
Specificity This antibody detects MAPKAP Kinase-5 (MAPKAPK5) pSer93.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S93 of Human MAPKAPK. Genename: MAPKAPK5
Isotype Ig

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling References for anti-MAPKAP Kinase 5 antibody (ABIN358154) Images back to top
Antigen
Alternative Name MAPKAP Kinase-5 (MAPKAPK5 Antibody Abstract)
Background The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.Synonyms: MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
Molecular Weight 54220 Da
Gene ID 8550, 9606
UniProt Q8IW41
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling References for anti-MAPKAP Kinase 5 antibody (ABIN358154) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details References for anti-MAPKAP Kinase 5 antibody (ABIN358154) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAPKAP Kinase 5 antibody (ABIN358154)

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling Images back to top
Background publications

New, Jiang, Zhao, Liu, Zhu, Flood, Kato, Parry, Han: "PRAK, a novel protein kinase regulated by the p38 MAP kinase." in: The EMBO journal, Vol. 17, Issue 12, pp. 3372-84, 1998

Ni, Wang, Diener, Yao: "MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase." in: Biochemical and biophysical research communications, Vol. 243, Issue 2, pp. 492-6, 1998

Images

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 antibody (ABIN358154) back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pSer93) antibody (ABIN358154) anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pSer93) antibody
 image for anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pSer93) antibody (ABIN358154) anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pSer93) antibody (Image 2)