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anti-Mouse (Murine) MAPKAP Kinase 5 antibody for Immunoprecipitation

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAP-K5
  • MAPKAPK5
  • MK-5
  • MK5
  • PRAK
Epitope
Internal Region
23
22
20
14
13
7
7
6
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
148
59
8
2
2
2
Host
Goat
101
44
19
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
139
86
41
14
12
10
8
3
2
1
1
1
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Available images

Catalog No. ABIN1590011
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008946489 ABIN2563873 IP ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0008946489 ABIN2563874 IP ELISA WB Goat IgG AA 192-206 Log in to see Polyclonal
0.0008946489 ABIN1590013 IP ELISA WB Goat Internal Region Log in to see Polyclonal
0.0008946489 ABIN3060848 IP WB Rabbit Log in to see Polyclonal

Similar anti-MAPKAP Kinase 5 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 44 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Epitope Internal Region
(23), (22), (20), (14), (13), (7), (7), (6), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(148), (59), (8), (2), (2), (2)
Host Goat
(101), (44), (19)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(139), (86), (41), (14), (12), (10), (8), (3), (2), (1), (1), (1)
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2816042
Sequence Peptide sequence: STEALDNVLP SAQ
Cross-Reactivity Human, Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Characteristics Peptide Lenght: 13

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MK5 / MAPKAPK5 (MAPKAPK5 Antibody Abstract)
Gene ID 8550, 17165, 498183
NCBI Accession NP_003659, NP_620777
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling Images back to top
Application Notes Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 µg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Images back to top
Storage -20 °C

Images

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling back to top
Supplier Images
 image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (Internal Region) (ABIN1590011) ABIN1590011 and EB11928 (1.5ug) immunoprecipitations from lysates of MK5/ERK3 double ...
 image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (Internal Region) (ABIN1590011) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane...