|Application / Reactivity||Cow (Bovine)||Dog (Canine)||Human||Mouse (Murine)||Rat (Rattus)||Non Human Primates|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies||7 Antibodies||6 Antibodies||6 Antibodies|
|Western Blotting (WB)||867 Antibodies||122 Antibodies||555 Antibodies||532 Antibodies||31 Antibodies||1 Antibodies|
|Immunostaining (ISt)||12 Antibodies|
|Immunoprecipitation (IP)||39 Antibodies||22 Antibodies||22 Antibodies||20 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||84 Antibodies||17 Antibodies|
|Antigen||Microtubule-Associated Protein tau (MAPT) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non Human Primates, Rat (Rattus) Alternatives|
|Conjugate||This MAPT antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-MAPT AntibodyTarget Details MAPT Application Details Handling References for anti-MAPT Antibody (ABIN372727) Images
|Specificity||Specific for ~59, 65, 68k Tau protein phosphorylated at Ser416.|
Species reactivity (expected):Human, Bovine, Canine, Mouse and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Affinity Chromatography via sequential chromatography on phospho- and dephospho-peptide affinity columns|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of Rat MAPT/TAU.|
Target Details MAPTProduct Details anti-MAPT Antibody Application Details Handling References for anti-MAPT Antibody (ABIN372727) Images back to top
|Alternative Name||MAPT / tau (MAPT Antibody Abstract)|
|Background||Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer's disease (AD), Pick's disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau|
|Pathways||MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size|
Application DetailsProduct Details anti-MAPT Antibody Target Details MAPT Handling References for anti-MAPT Antibody (ABIN372727) Images back to top
Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPT Antibody Target Details MAPT Application Details References for anti-MAPT Antibody (ABIN372727) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-MAPT Antibody (ABIN372727)Product Details anti-MAPT Antibody Target Details MAPT Application Details Handling Images back to top
Binder, Frankfurter, Rebhun: "The distribution of tau in the mammalian central nervous system." in: The Journal of cell biology, Vol. 101, Issue 4, pp. 1371-8, 1985
ImagesProduct Details anti-MAPT Antibody Target Details MAPT Application Details Handling References for anti-MAPT Antibody (ABIN372727) back to top