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anti-Cat (Feline) MCL-1 antibody for Western Blotting

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Epitope
C-Term, Isoform 1
34
23
23
16
15
14
13
13
11
10
9
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
331
110
72
9
9
5
5
3
3
1
1
1
1
Host
Goat
223
98
19
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
7
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
282
142
114
86
83
52
32
27
25
22
7
6
1
Supplier
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Catalog No. ABIN374436
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN126984 IHC (p) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-MCL-1 antibody for Western Blotting

Similar anti-MCL-1 Antibodies

Application / Reactivity Human Cat (Feline) Dog (Canine) Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 276 Antibodies 2 Antibodies 9 Antibodies 72 Antibodies 50 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies 24 Antibodies 7 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies 1 Antibodies 1 Antibodies 16 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 114 Antibodies 22 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope C-Term, Isoform 1
(34), (23), (23), (16), (15), (14), (13), (13), (11), (10), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
(331), (110), (72), (9), (9), (5), (5), (3), (3), (1), (1), (1), (1)
Host Goat
(223), (98), (19), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(282), (142), (114), (86), (83), (52), (32), (27), (25), (22), (7), (6), (1)
Pubmed 1 reference available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN374436) Images
Sequence C-AGVGAGLAYL IR
Specificity This antibody is expected to recognise isoform 1 (NP_068779.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog) and Feline (Cat).
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide from the C Terminus of the protein sequence according to NP_068779.1

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN374436) Images back to top
Antigen
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004
Gene ID 4170
NCBI Accession NP_068779
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN374436) Images back to top
Application Notes Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of Human Lung and cellline K562 (calculated MW of 37.5 kDa according to NP_068779.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN374436) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MCL-1 Antibody (ABIN374436)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Background publications

Opferman, Letai, Beard, Sorcinelli, Ong, Korsmeyer: "Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1." in: Nature, Vol. 426, Issue 6967, pp. 671-6, 2003

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN374436) back to top
Supplier Images
 image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term) (ABIN374436) anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term), (Isoform 1) antibody