anti-Cat (Feline) MCL-1 antibody for Western Blotting

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
  • myeloid cell leukemia sequence 1
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • Mcl1
  • MCL1
Epitope
C-Term, Isoform 1
30
22
18
17
15
15
14
13
12
9
7
6
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
327
125
81
13
12
10
8
3
3
2
2
1
1
1
Host
Goat
216
98
21
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
6
5
5
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
279
139
115
92
79
44
31
30
26
25
6
5
5
1
Supplier
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Catalog No. ABIN5552970
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126984 IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN605039 IHC (p) IP WB Rabbit Log in to see Polyclonal

Similar anti-MCL-1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cat (Feline) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 2 Antibodies 1 Antibodies
Western Blotting (WB) 272 Antibodies 3 Antibodies 12 Antibodies 87 Antibodies 54 Antibodies
Immunoprecipitation (IP) 2 Antibodies 3 Antibodies 28 Antibodies 10 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 92 Antibodies 2 Antibodies 4 Antibodies 22 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope C-Term, Isoform 1
(30), (22), (18), (17), (15), (15), (14), (13), (12), (9), (7), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(327), (125), (81), (13), (12), (10), (8), (3), (3), (2), (2), (1), (1), (1)
Host Goat
(216), (98), (21), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(279), (139), (115), (92), (79), (44), (31), (30), (26), (25), (6), (5), (5), (1)
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling Images
Sequence C-AGVGAGLAYL IR
Specificity This antibody is expected to recognise isoform 1 (NP_068779.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog) and Feline (Cat).
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide from the C Terminus of the protein sequence according to NP_068779.1

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004
Gene ID 4170
NCBI Accession NP_068779
UniProt Q07820
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling Images back to top
Application Notes Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of Human Lung and cellline K562 (calculated MW of 37.5 kDa according to NP_068779.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.