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anti-Rat (Rattus) PAK2 antibody for Enzyme Immunoassay

Recommended PAK2 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 2 (PAK2) Antibodies
  • pak2
  • cb422
  • kinase
  • zgc:91798
  • wu:fb71h01
  • si:dz198m22.1
  • pak65
  • pakgamma
  • PAK65
  • PAKgamma
  • 5330420P17Rik
  • A130002K10Rik
  • AI836325
  • D16Ertd269e
  • PAK-2
  • mKIAA4182
  • gamma-PAK
  • PAKI
  • Pak3
  • p21 (CDKN1A)-activated kinase 2a
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • pak2a
  • pak2
  • PAK2
  • Pak2
Epitope
N-Term
39
21
12
8
6
5
5
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
173
94
89
12
8
1
Host
Rabbit
155
18
Clonality
Polyclonal
Conjugate
This PAK2 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
99
76
29
18
6
6
4
2
2
2
1
Supplier
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Catalog No. ABIN500441
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN500439 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 4

Top referenced anti-PAK2 antibody for Enzyme Immunoassay

  • Human Polyclonal PAK2 Primary Antibody for EIA, IHC (p) - ABIN500441 (4 Publications): Jaffer, Chernoff: p21-activated kinases: three more join the Pak. in The international journal of biochemistry & cell biology 2002 (PubMed)

  • Human Polyclonal PAK2 Primary Antibody for EIA, IHC (p) - ABIN500439 (4 Publications): Ang, Patel, Yin, Sundaram, Fritchie, Zhao, Liu, Freemerman, Wilkerson, Walter, Weissler, Shockley, Couch, Zanation, Hackman, Chera, Harris, Miller, Thorne, Hayward, Funkhouser, Olshan, Shores, Makowski, Hayes: High XRCC1 protein expression is associated with poorer survival in patients with head and neck squamous cell carcinoma. in Clinical cancer research : an official journal of the American Association for Cancer Research 2011 (PubMed)

Similar anti-PAK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 58 Antibodies 58 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 5 Antibodies 5 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 38 Antibodies 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 78 Antibodies

General

Antigen P21-Activated Kinase 2 (PAK2) Antibodies
Epitope N-Term
(39), (21), (12), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(173), (94), (89), (12), (8), (1)
Host Rabbit
(155), (18)
Clonality
Polyclonal
Conjugate This PAK2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(158), (99), (76), (29), (18), (6), (6), (4), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-PAK2 Antibody

Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN500441) Images
Specificity This antibody detects PAK2 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen 14 amino acid peptide from near the amino terminus of human PAK2
Isotype IgG

Target Details PAK2

Product Details anti-PAK2 Antibody Application Details Handling References for anti-PAK2 Antibody (ABIN500441) Images back to top
Antigen
Alternative Name PAK2 (PAK2 Antibody Abstract)
Background The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58
Gene ID 5062
NCBI Accession NP_002568
UniProt Q13177
Research Area Cancer, Apoptosis/Necrosis, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha

Application Details

Product Details anti-PAK2 Antibody Target Details PAK2 Handling References for anti-PAK2 Antibody (ABIN500441) Images back to top
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details References for anti-PAK2 Antibody (ABIN500441) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-PAK2 Antibody (ABIN500441)

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling Images back to top
Background publications

Ang, Patel, Yin, Sundaram, Fritchie, Zhao, Liu, Freemerman, Wilkerson, Walter, Weissler, Shockley, Couch, Zanation, Hackman, Chera, Harris, Miller, Thorne, Hayward, Funkhouser, Olshan, Shores, Makowski, Hayes: "High XRCC1 protein expression is associated with poorer survival in patients with head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6542-52, 2011

Vilas, Corvi, Plummer, Seime, Lambkin, Berthiaume: "Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6542-7, 2006

Jaffer, Chernoff: "p21-activated kinases: three more join the Pak." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 7, pp. 713-7, 2002

Rudel, Bokoch: "Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2." in: Science (New York, N.Y.), Vol. 276, Issue 5318, pp. 1571-4, 1997

Images

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN500441) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PAK2 antibody (P21-Activated Kinase 2) (N-Term) (ABIN500441) Immunohistochemistry of PAK2 in mouse spleen tissue with this product at 10 μg/ml.
Western Blotting (WB) image for anti-PAK2 antibody (P21-Activated Kinase 2) (N-Term) (ABIN500441) Western blot analysis of PAK2 in Jurkat lysate with this product at (A) 0.5, (B) 1 an...