anti-Cow (Bovine) RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
Middle Region
73
72
47
40
39
37
28
28
28
27
22
22
22
20
19
18
17
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
808
410
385
24
23
16
15
13
8
7
7
5
4
3
2
1
1
1
1
Host
Rabbit
769
46
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
489
348
202
172
156
104
50
34
19
18
17
7
2
2
2
1
Options
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Catalog No. ABIN2792055
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3484275 ABIN2957891 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.3484275 ABIN2705627 IC IF WB Rabbit Center Log in to see Polyclonal
1 ABIN5551625 WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN5551626 WB Rabbit IgG pSer642 Log in to see Polyclonal
1 ABIN318805 WB Rabbit IgG pSer642 Log in to see Polyclonal
1 ABIN2476311 WB Rabbit IgG pSer642 Log in to see Polyclonal 2
1 ABIN295906 IP WB Sheep IgG AA 657-668 Log in to see Polyclonal
1 ABIN2755776 WB Rabbit N-Term, pSer43 Log in to see Polyclonal
1 ABIN2755777 IHC WB Rabbit N-Term Log in to see Polyclonal
1 ABIN602224 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN602225 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN597445 WB Rabbit IgG Log in to see Polyclonal
1 ABIN209208 WB Rat IgG1 AA 334-347, pSer338 Log in to see
1 ABIN2627495 ICC IF WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1929029 IP WB Rabbit IgG AA 598-648 Log in to see Polyclonal
1 ABIN303955 WB Rabbit pSer642 Log in to see Polyclonal
1 ABIN1582164 ELISA WB Rabbit pSer642 Log in to see Polyclonal
1 ABIN1582169 ELISA WB Rabbit pSer301 Log in to see Polyclonal
1 ABIN2959896 WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN2959895 WB Rabbit IgG pSer642 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Horse (Equine) Rabbit Zebrafish Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 6 Antibodies 6 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 464 Antibodies 256 Antibodies 234 Antibodies 24 Antibodies 16 Antibodies 8 Antibodies 8 Antibodies 4 Antibodies 2 Antibodies
RNA Interference (RNAi) 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope Middle Region
(73), (72), (47), (40), (39), (37), (28), (28), (28), (27), (22), (22), (22), (20), (19), (18), (17), (15), (15), (15), (15), (15), (14), (14), (10), (8), (8), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(808), (410), (385), (24), (23), (16), (15), (13), (8), (7), (7), (5), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(769), (46), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(489), (348), (202), (172), (156), (104), (50), (34), (19), (18), (17), (7), (2), (2), (2), (1)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2185168
Sequence SGTQEKNKIR PRGQRDSSYY WEIEASEVML STRIGSGSFG TVYKGKWHGD
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against RAF1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of Human RAF1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Protein Interaction Partner: Prkab1, FYCO1, NPLOC4, TMEM70, COPS7A, DCAF8, PACSIN3, YWHAQ, RANBP9, RB1, PURA, MAP2K2, MAP2K1, MAPK3, MAPK1, PRKAR1A, MYBPC1, HSP90AA1, AKT1, FAM96B, MRAS, HUWE1, SHOC2, UBC, CDC37, IRS4, GPRASP2, STUB1, SH3KBP1, HIPK4, CDK20, YWHAB, TBXA2R, KRAS, YWHAE
Protein Size: 567
Molecular Weight 62 kDa
Gene ID 5894
NCBI Accession XP_005265415
UniProt B4E0X2
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (Middle Region) (ABIN2792055) Host: Rabbit Target Name: RAF1 Sample Type: Jurkat Whole Cell lysates Antibody Diluti...