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anti-Human RAF1 antibody for Flow Cytometry

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Reactivity
Human
787
337
319
18
18
10
5
4
4
2
2
2
1
1
Host
Mouse
739
38
16
7
2
Clonality
Monoclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
457
351
204
181
132
79
39
29
18
16
11
2
2
2
Supplier
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Catalog No. ABIN969560
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
0.30464333 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
0.30464333 ABIN2491387 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.30464333 ABIN2491390 FACS IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.30464333 ABIN1844891 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.30464333 ABIN1846392 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0010463598 ABIN392261 FACS IF IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.0010463598 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
0.0010463598 ABIN1500582 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4
0.0010463598 ABIN1500583 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4
0.0010463598 ABIN1929044 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN1929045 FACS IHC ELISA WB Biotin Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN1929043 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN1929047 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN1929046 FACS IHC ELISA WB FITC Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN1929048 FACS IHC ELISA WB HRP Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN2347799 FACS IHC ELISA WB Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN2347801 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN2347803 FACS IHC ELISA WB FITC Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal
0.0010463598 ABIN2347805 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 85-113 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Flow Cytometry

Similar anti-RAF1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 181 Antibodies
ELISA 350 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies
Immunohistochemistry (IHC) 204 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 448 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Reactivity Human
(787), (337), (319), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Mouse
(739), (38), (16), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(457), (351), (204), (181), (132), (79), (39), (29), (18), (16), (11), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN969560) Images
Purification Purified
Immunogen Purified recombinant fragment of human RAF1 expressed in E. coli.
Clone 4G4
Isotype IgG1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN969560) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Molecular Weight 73 kDa
Gene ID 5894
HGNC 5894
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN969560) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN969560) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-RAF1 Antibody (ABIN969560)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Takezawa, Okamoto, Yonesaka, Hatashita, Yamada, Fukuoka, Nakagawa: "Sorafenib inhibits non-small cell lung cancer cell growth by targeting B-RAF in KRAS wild-type cells and C-RAF in KRAS mutant cells." in: Cancer research, Vol. 69, Issue 16, pp. 6515-21, 2009

Peng, Guo, Ji, Ren, Yang, Li, Qu, Li, Tang, Yan, Tang: "Sorafenib induces growth inhibition and apoptosis in human synovial sarcoma cells via inhibiting the RAF/MEK/ERK signaling pathway." in: Cancer biology & therapy, Vol. 8, Issue 18, pp. 1729-36, 2009

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN969560) back to top
Supplier Images
ELISA image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN969560) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN969560) Figure 2: Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and ne...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN969560) Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN969560) Figure 4: Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG...
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN969560) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...