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anti-Mouse (Murine) RAF1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
AA 25-60
74
60
56
39
36
34
27
26
26
26
23
23
20
20
19
17
17
16
16
15
15
14
13
13
12
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
779
331
313
18
18
10
5
4
4
2
2
1
1
Host
Rabbit
729
38
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
447
351
199
181
131
74
37
29
18
16
9
2
2
2
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN733208
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008812695 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.0008812695 ABIN879527 IF (p) Alexa Fluor 488 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN879528 IF (p) Alexa Fluor 350 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN879529 IF (p) Alexa Fluor 555 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN879530 IF (p) Alexa Fluor 647 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN733631 IF (p) Cy3 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN733632 IF (p) Cy5 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN733635 IF (p) FITC Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN733634 IF (p) Cy7 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN733633 IF (p) Cy5.5 Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN2810550 IF (p) Alexa Fluor 594 Rabbit IgG AA 310-360, Tyr340, pSer338 Log in to see Polyclonal
0.0008812695 ABIN875646 IF (p) Alexa Fluor 488 Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.0008812695 ABIN875647 IF (p) Alexa Fluor 350 Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.0008812695 ABIN875648 IF (p) Alexa Fluor 555 Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.0008812695 ABIN875649 IF (p) Alexa Fluor 647 Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.0008812695 ABIN885887 IF (p) Alexa Fluor 647 Rabbit IgG AA 25-60 Log in to see Polyclonal
0.0008812695 ABIN885888 IF (p) Alexa Fluor 350 Rabbit IgG AA 25-60 Log in to see Polyclonal
0.0008812695 ABIN885889 IF (p) Alexa Fluor 488 Rabbit IgG AA 25-60 Log in to see Polyclonal
0.0008812695 ABIN885890 IF (p) Alexa Fluor 555 Rabbit IgG AA 25-60 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 85 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 20 Antibodies 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 121 Antibodies 121 Antibodies
Immunohistochemistry (IHC) 72 Antibodies 82 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 50 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 186 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope AA 25-60
(74), (60), (56), (39), (36), (34), (27), (26), (26), (26), (23), (23), (20), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (12), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(779), (331), (313), (18), (18), (10), (5), (4), (4), (2), (2), (1), (1)
Host Rabbit
(729), (38), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(447), (351), (199), (181), (131), (74), (37), (29), (18), (16), (9), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN733208) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human c Raf
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN733208) Images back to top
Antigen
Alternative Name C-Raf/Raf1 (RAF1 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Subcellular location: Cytoplasm, Nucleus, Mitochondrion, Cell membrane


Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF
Gene ID 5894
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN733208) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN733208) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN733208)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Wang, Fu, Jiang, Yu, Wang, Han, Liu, Wang: "Bone marrow mononuclear cell transplantation promotes therapeutic angiogenesis via upregulation of the VEGF-VEGFR2 signaling pathway in a rat model of vascular dementia." in: Behavioural brain research, Vol. 265, pp. 171-80, 2014

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN733208) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 25-60) (ABIN733208) Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 ...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 25-60) (ABIN733208) Images provided the Independent Validation Program (badge number 29651)Formalin-fixed...
'Independent Validation' Badge
Lot Number 130909
Method validated Immunohistochemistry
Positive Control Human colon villus epithelial cells
Negative Control Human liver
Notes Raf1 exhibits uniformly low expression across all human tissues; higher expression found in colon, lower in liver. Tissue-specific expression is detected in colon, with expected low level expression in liver.
Primary Antibody
  • Antigen: V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)
  • Catalog number: ABIN733208
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130909
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Human colon tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human colon tissue stained with isotype control
  • Secondary only control: Human colon tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 1: Human colon tissue stained with anti-RAF1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 2: Human liver tissue stained with anti-RAF1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 3: Human colon tissue stained with isotype control antibody (brown) and counte...
Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 25-60) antibody (ABIN733208) Figure 4: Human colon tissue stained with secondary atnibody only (brown) and counter...