anti-Mouse (Murine) RPS6KA1 antibody for ELISA

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • Rsk1
  • p90rsk
  • rsk
  • HU-1
  • MAPKAPK1A
  • RSK
  • RSK1
  • RPS6KA
  • hu-1
  • mapkapk1a
  • rsk1
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MRX19
  • RSK2
  • S6K-alpha3
  • p90-RSK2
  • pp90RSK2
  • ribosomal protein S6 kinase polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Rps6ka1
  • RPS6KA1
  • rps6ka1
  • RPS6KA3
Epitope
C-Term
59
45
37
28
27
25
17
12
10
9
8
8
8
8
6
5
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Horse (Equine), Cow (Bovine), Dog (Canine)
346
184
152
34
15
10
8
6
6
4
4
2
2
1
1
1
1
Host
Rabbit
343
42
Clonality
Polyclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
21
20
18
12
12
12
6
6
5
5
5
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
278
160
97
62
61
52
43
43
19
7
2
1
1
1
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Catalog No. ABIN1043904
$ 379.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1043905 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN361702 ELISA ICC IF IP WB Rabbit C-Term Log in to see Polyclonal 4
1 ABIN964912 ELISA WB Rabbit IgG pSer732, C-Term Log in to see Polyclonal
1 ABIN2486884 ELISA ICC IF IP WB Atto 390 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486885 ELISA ICC IF IP WB Atto 488 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486886 ELISA ICC IF IP WB Atto 565 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486887 ELISA ICC IF IP WB Atto 594 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486888 ELISA ICC IF IP WB Atto 633 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486889 ELISA ICC IF IP WB Atto 655 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486891 ELISA ICC IF IP WB Atto 700 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486892 ELISA ICC IF IP WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486893 ELISA ICC IF IP WB APC Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486894 ELISA ICC IF IP WB Biotin Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486895 ELISA ICC IF IP WB FITC Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486896 ELISA ICC IF IP WB HRP Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486897 ELISA ICC IF IP WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486898 ELISA ICC IF IP WB PerCP Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486899 ELISA ICC IF IP WB PE Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486900 ELISA ICC IF IP WB Streptavidin Rabbit C-Term Log in to see Polyclonal 4
1 ABIN2486890 ELISA ICC IF IP WB Atto 680 Rabbit C-Term Log in to see Polyclonal 4

Top referenced anti-RPS6KA1 antibody for ELISA

Similar anti-RPS6KA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 34 Antibodies 252 Antibodies 133 Antibodies 104 Antibodies 13 Antibodies 9 Antibodies
Immunoprecipitation (IP) 24 Antibodies 43 Antibodies 32 Antibodies 32 Antibodies 5 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 56 Antibodies 29 Antibodies 28 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope C-Term
(59), (45), (37), (28), (27), (25), (17), (12), (10), (9), (8), (8), (8), (8), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Horse (Equine), Cow (Bovine), Dog (Canine)
(346), (184), (152), (34), (15), (10), (8), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(343), (42)
Clonality
Polyclonal
Conjugate This RPS6KA1 antibody is un-conjugated
(21), (20), (18), (12), (12), (12), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(278), (160), (97), (62), (61), (52), (43), (43), (19), (7), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904) Images
Specificity Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Characteristics Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Sterility Sterile filtered
Immunogen Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Immunogen Type: Peptide
Isotype IgG

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904) Images back to top
Antigen
Alternative Name p90 RSK1 (RPS6KA1 Antibody Abstract)
Background Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Synonyms: p90 RSK1, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPKAPK1A, MAPKAP kinase 1a, MAPKAPK-1a
Gene ID 6195
NCBI Accession NP_001006666
UniProt Q15418
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904) Images back to top
Application Notes Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.
Comment

Gene Name: RPS6KA1

Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904) Images back to top
Format Liquid
Concentration 1.51 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904)

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Product cited in:

Chaturvedi, Poppleton, Stringfield, Barbier, Patel: "Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4586-600, 2006

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling ProductDetails: References for anti-RPS6KA1 antibody (ABIN1043904) back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (C-Term) (ABIN1043904) Western Blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysa...