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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||39 Antibodies||55 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||3 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||15 Antibodies||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies||44 Antibodies|
|Immunohistochemistry (IHC)||19 Antibodies||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies||20 Antibodies|
|Immunoprecipitation (IP)||25 Antibodies||24 Antibodies|
|Western Blotting (WB)||74 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KA1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-RPS6KA1 AntibodyTarget Details RPS6KA1 Application Details Handling Images
|Immunogen||A synthetic peptide corresponding to residues in N-terminus of human p90-RSK1 was used as immunogen.|
Target Details RPS6KA1Product Details anti-RPS6KA1 Antibody Application Details Handling Images back to top
|Alternative Name||RPS6KA1 (RPS6KA1 Antibody Abstract)|
Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases,which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonineprotein kinases (1) activated in response to mitogenic stimuli, including extracellularsignal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK invitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role intranscriptional regulation by translocating to the nucleus and phosphorylating c-Fos andCREB (2,4). Alternate Names: anti-90 kDa ribosomal protein S6 kinase 1 antibody, anti-dJ590P13.1 antibody, anti-HU1 antibody, anti-HU1 antibody, anti-MAP kinase activated protein kinase 1a antibody,anti-MAPKAPK1A antibody, anti-p90 RSK1 antibody, anti-p90S6K antibody,anti-pp90RSK1 antibody, anti-Ribosomal protein S6 kinase 90kD 1 antibody,anti-Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, anti-Ribosomal proteinS6 kinase 90kDa polypeptide 1 antibody, anti-Ribosomal protein S6 kinase alpha 1antibody, anti-Ribosomal S6 kinase 1 antibody, anti-Rps6ka1 antibody, anti-RSK 1antibody, anti-RSK 1 p90 antibody, anti-RSK antibody, anti-RSK1 antibody, anti-S6Kalpha 1 antibody.
Gene Symbol: RPS6KA1
|Gene ID||6195, 20111, 81771|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 83kDa.
Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 1:10, Western Blot 1:1000
Protocol specific for RSK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling back to top