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anti-Human RPS6KA3 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended RPS6KA3 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MPK-9
  • MRX19
  • p90-RSK2
  • pp90RSK2
  • RSK
  • Rsk2
  • RSK2
  • S6K-alpha3
Epitope
AA 210-260, pSer227
22
14
13
13
13
7
6
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
58
51
7
7
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
120
25
Clonality
Polyclonal
Conjugate
This RPS6KA3 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
97
44
25
23
22
18
13
8
7
5
3
2
2
Supplier
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Catalog No. ABIN745268
$ 291.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008846439 ABIN745283 IF (p) IHC (p) WB Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN700720 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN2808934 IF (p) Alexa Fluor 594 Rabbit IgG AA 510-550, pTyr529 Log in to see Polyclonal
0.0008846439 ABIN879843 IF (p) Alexa Fluor 647 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN879842 IF (p) Alexa Fluor 555 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN879845 IF (p) Alexa Fluor 350 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN879841 IF (p) Alexa Fluor 488 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN745286 IF (p) Cy3 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN745288 IF (p) Cy5.5 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN745287 IF (p) Cy5 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN745290 IF (p) FITC Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN745289 IF (p) Cy7 Rabbit IgG pTyr529 Log in to see Polyclonal
0.0008846439 ABIN914089 IF (p) Alexa Fluor 647 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN914088 IF (p) Alexa Fluor 350 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN914091 IF (p) Alexa Fluor 555 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN914092 IF (p) Alexa Fluor 488 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN700723 IF (p) Cy3 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN700724 IF (p) Cy5 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN700725 IF (p) Cy5.5 Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0008846439 ABIN700727 IF (p) FITC Rabbit IgG AA 80-130 Log in to see Polyclonal

Similar anti-RPS6KA3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
Immunocytochemistry (ICC) 2 Antibodies 3 Antibodies
Immunofluorescence (IF) 2 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 8 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 17 Antibodies
Immunoprecipitation (IP) 3 Antibodies 8 Antibodies
Western Blotting (WB) 33 Antibodies 37 Antibodies
ELISA 1 Antibodies 44 Antibodies
Dot Blot (DB) 18 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies
Epitope AA 210-260, pSer227
(22), (14), (13), (13), (13), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (58), (51), (7), (7), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(120), (25)
Clonality
Polyclonal
Conjugate This RPS6KA3 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(97), (44), (25), (23), (22), (18), (13), (8), (7), (5), (3), (2), (2)
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Product Details anti-RPS6KA3 Antibody

Target Details RPS6KA3 Application Details Handling Images
Specificity This antibody will cross react with Ribosomal protein S6 kinase alpha-1 when phosphorylated at Ser221 and Ribosomal protein S6 kinase alpha-6 at Ser232.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human RSK2 around the phosphorylation site of Ser227
Isotype IgG

Target Details RPS6KA3

Product Details anti-RPS6KA3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rsk2 (RPS6KA3 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4).

Subcellular location: Cytoplasm, Nucleus


Synonyms: Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated protein kinase 1, ISPK-1, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, Ribosomal S6 kinase 2, RSK-2, pp90RSK2, RPS6KA3, ISPK1, MAPKAPK1B, RSK2
Gene ID 6197
UniProt P51812
Research Area Signaling
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (AA 210-260) (ABIN745268) Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-RSK2 (Ser227...
Immunohistochemistry (IHC) image for anti-RPS6KA3 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3) (AA 210-260) (ABIN745268) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-R...