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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||26 Antibodies||24 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||59 Antibodies|
|Antigen||serum/glucocorticoid Regulated Kinase 1 (SGK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SGK1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-SGK1 AntibodyTarget Details SGK1 Application Details Handling Images
|Immunogen||A synthetic peptide corresponding to residues of human SGK was used as an immunogen.|
Target Details SGK1Product Details anti-SGK1 Antibody Application Details Handling Images back to top
|Alternative Name||SGK1 / SGK (SGK1 Antibody Abstract)|
Serum and glucocorticoid-regulated kinase (SGK-1) is a 50kDa Serine/Threonine kinasethat plays a role in the regulation of epithelial Na+ transport. Two additional SGKisoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylationat Thr256 and Ser422 by the phosphatidyl-dependent protein kinase-1 (PDK1) andPDK-2 respectively (1-2). Nedd4-2 regulates SGK activity by catalyzing its ubiquinationand degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+transport (4). While environmental stresses increases its expression, SGK is known toplay a role in promoting cell survival, proliferation and differentiation (5). Alternate Names: anti-Serine/threonine protein kinase SGK antibody, anti-Serine/threonine protein kinaseSgk1 antibody, anti-Serum and glucocorticoid regulated kinase antibody,anti-Serum/glucocorticoid regulated kinase 1 antibody, anti-Serum/glucocorticoidregulated kinase antibody, anti-SGK 1 antibody, anti-SGK antibody.
Gene Symbol: SGK1
|Gene ID||6446, 20393, 29517|
|Pathways||MAPK Signaling, Notch Signaling|
Application DetailsProduct Details anti-SGK1 Antibody Target Details SGK1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 50-55kDa.
Recommended dilutions: Flow Cytometry 1:30, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:50, Western Blot 1:500
Protocol specific for SGK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-SGK1 Antibody Target Details SGK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-SGK1 Antibody Target Details SGK1 Application Details Handling back to top