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anti-Human SGK1 antibody for Flow Cytometry

Recommended SGK1 Antibody (supplied by: Log in to see )

Antigen
serum/glucocorticoid Regulated Kinase 1 (SGK1) Antibodies
  • cb1083
  • sgk
  • SGK
  • Sgk
  • wu:fc20a09
Reactivity
Human, Mouse (Murine), Rat (Rattus)
210
95
91
9
5
5
4
2
2
1
Host
Rabbit
159
42
8
4
Clonality
Monoclonal
Conjugate
This SGK1 antibody is un-conjugated
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
165
100
73
49
39
33
15
14
6
5
2
2
1
1
Supplier
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Available images

Catalog No. ABIN258464
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666476 ABIN2359478 FACS IP IHC WB Rabbit IgG Log in to see 9i206
0.0010666476 ABIN2744332 FACS IHC WB Rabbit AA 194-431 Log in to see Polyclonal

Similar anti-SGK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 26 Antibodies 25 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 10 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 59 Antibodies

General

Antigen serum/glucocorticoid Regulated Kinase 1 (SGK1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(210), (95), (91), (9), (5), (5), (4), (2), (2), (1)
Host Rabbit
(159), (42), (8), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SGK1 antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(165), (100), (73), (49), (39), (33), (15), (14), (6), (5), (2), (2), (1), (1)
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Product Details anti-SGK1 Antibody

Target Details SGK1 Application Details Handling Images
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues of human SGK was used as an immunogen.
Clone Y238
Isotype IgG

Target Details SGK1

Product Details anti-SGK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SGK1 / SGK (SGK1 Antibody Abstract)
Background Serum and glucocorticoid-regulated kinase (SGK-1) is a 50kDa Serine/Threonine kinasethat plays a role in the regulation of epithelial Na+ transport. Two additional SGKisoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylationat Thr256 and Ser422 by the phosphatidyl-dependent protein kinase-1 (PDK1) andPDK-2 respectively (1-2). Nedd4-2 regulates SGK activity by catalyzing its ubiquinationand degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+transport (4). While environmental stresses increases its expression, SGK is known toplay a role in promoting cell survival, proliferation and differentiation (5). Alternate Names: anti-Serine/threonine protein kinase SGK antibody, anti-Serine/threonine protein kinaseSgk1 antibody, anti-Serum and glucocorticoid regulated kinase antibody,anti-Serum/glucocorticoid regulated kinase 1 antibody, anti-Serum/glucocorticoidregulated kinase antibody, anti-SGK 1 antibody, anti-SGK antibody.
Gene Symbol: SGK1
Gene ID 6446, 20393, 29517
Pathways MAPK Signaling, Notch Signaling

Application Details

Product Details anti-SGK1 Antibody Target Details SGK1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 50-55kDa.
Recommended dilutions: Flow Cytometry 1:30, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:50, Western Blot 1:500
Protocol Protocol specific for SGK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-SGK1 Antibody Target Details SGK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-SGK1 Antibody Target Details SGK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SGK1 antibody (serum/glucocorticoid Regulated Kinase 1) (ABIN258464) Western blot analysis of anti-SGK RabMAb (cat. #110-57566) 1:500 dilution A- A431 B- ...
Immunohistochemistry (IHC) image for anti-SGK1 antibody (serum/glucocorticoid Regulated Kinase 1) (ABIN258464) Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-S...