SGK1 antibody (Ser78)

Catalog No. ABIN6243740

Product Details anti-SGK1 Antibody

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Binding Specificity: AA 70-103, Ser78

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Predicted Reactivity: B, C, Pr, M, Rb, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SGK (Ser78) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 70-103 amino acids from the human region of human SGK (Ser78).

Clone: RB57649

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK (SGK1 Products)

Synonyms: SGK antibody, Sgk antibody, sgk-B antibody, sgk2 antibody, cb1083 antibody, sgk antibody, wu:fc20a09 antibody, sgk-A antibody, sgk1 antibody, sgk1-a antibody, sgk1-b antibody, serum/glucocorticoid regulated kinase 1 antibody, Serine/threonine-protein kinase sgk-1 antibody, serum/glucocorticoid regulated kinase 1 S homeolog antibody, serum/glucocorticoid regulated kinase 1 L homeolog antibody, SGK1 antibody, Sgk1 antibody, sgk-1 antibody, sgk1.S antibody, sgk1 antibody, sgk1.L antibody

Background: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na(+) retention, renal K(+) elimination, salt appetite, gastric acid secretion, intestinal Na(+)/H(+) exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na(+) channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K(+) channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na(+)/K(+) ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na(+) transport than isoform 1.

Molecular Weight: 48942

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway