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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||26 Antibodies||24 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||59 Antibodies|
|Antigen||serum/glucocorticoid Regulated Kinase 1 (SGK1) Antibodies|
|Epitope||AA 27-44, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SGK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-SGK1 AntibodyTarget Details SGK1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Sgk1(SGK1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: serum/glucocorticoid regulated kinase 1
Protein Name: Serine/threonine-protein kinase Sgk1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human SGK1(27-44aa FMKQRRMGLNDFIQKIAN), different from the related rat and mouse sequences by one amino acid.|
Target Details SGK1Product Details anti-SGK1 Antibody Application Details Handling Images back to top
|Alternative Name||SGK1 (SGK1 Antibody Abstract)|
|Background||Serine/threonine-protein kinase Sgk1, also called SGK1, encodes a serine/threonine protein kinase that plays an important role in cellular stress response. By fluorescence in situ hybridization, this gene is mapped to 6q23.2. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.|
|Research Area||Embryogenesis, Embryonic stem cells|
|Pathways||MAPK Signaling, Notch Signaling|
Application DetailsProduct Details anti-SGK1 Antibody Target Details SGK1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SGK1 Antibody Target Details SGK1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-SGK1 Antibody Target Details SGK1 Application Details Handling back to top