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anti-Human SIRT1 antibody for Immunocytochemistry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Reactivity
Human
293
138
70
21
13
11
8
7
7
6
5
4
4
3
2
2
2
1
Host
Mouse
195
85
26
6
3
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
15
13
10
7
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
249
128
81
63
60
47
40
36
33
7
5
4
4
2
2
2
1
Options
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Catalog No. ABIN969396
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.705893 ABIN4899186 FACS ICC Mouse IgG1 AA 1-747 Log in to see 834918
1 ABIN2668278 ICC IF IP WB Mouse IgG1 AA 581-630 Log in to see 2G1-F7 2
1 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
1 ABIN4353903 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
1 ABIN1500927 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 1F3
1 ABIN4880459 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 1F3
1 ABIN4353899 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-550, Internal Region Log in to see Polyclonal
1 ABIN4353902 ELISA ICC IF WB Chicken N-Term Log in to see Polyclonal
1 ABIN3201829 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 244-498 Log in to see
1 ABIN1176381 ICC IHC IP WB Rabbit IgG AA 244-498 Log in to see Polyclonal
1 ABIN1935168 ICC WB FITC Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935167 ICC WB Biotin Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935165 ICC WB Alkaline Phosphatase (AP) Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935169 ICC WB PE Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935166 ICC WB APC Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935170 ICC WB HRP Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN4353900 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3
1 ABIN4963877 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see 1F3
1 ABIN4963876 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see 1F3
1 ABIN4963881 ELISA ICC IF IHC IHC (p) WB Biotin Mouse IgG1 Log in to see 1F3

Top referenced anti-SIRT1 antibody for Immunocytochemistry

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Application / Reactivity Human
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Enzyme Immunoassay (EIA) 4 Antibodies
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Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 45 Antibodies
Immunofluorescence (IF) 62 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
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General

Antigen Sirtuin 1 (SIRT1) Antibodies
Reactivity Human
(293), (138), (70), (21), (13), (11), (8), (7), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(195), (85), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(15), (13), (10), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(249), (128), (81), (63), (60), (47), (40), (36), (33), (7), (5), (4), (4), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images
Purification Ascites
Immunogen Purified recombinant fragment of human SIRT1 expressed in E. Coli.
Clone 1F3
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molecular Weight 120 kDa
Gene ID 23411
HGNC 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-SIRT1 Antibody (ABIN969396)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, 2009

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 1: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 4: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), ...