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anti-Human SIRT1 antibody for Immunocytochemistry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Reactivity
Human, Monkey
280
125
64
21
8
8
6
4
3
3
2
2
2
2
1
1
Host
Mouse
186
79
27
7
3
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
15
13
10
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
233
129
74
63
61
47
34
29
27
5
5
4
4
2
2
1
Options
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Catalog No. ABIN969396
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.375843 ABIN4899186 FACS ICC Mouse IgG1 AA 1-747 Log in to see 834918
26.375843 ABIN1846081 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN2668278 ICC IF IP WB Mouse IgG1 AA 581-630 Log in to see 2G1-F7 2
1 ABIN1500927 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 1F3
1 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
1 ABIN4353903 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
1 ABIN4880459 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 1F3
1 ABIN3201829 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 244-498 Log in to see
1 ABIN1176381 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 244-498 Log in to see Polyclonal
1 ABIN1935167 ICC WB Biotin Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935165 ICC WB Alkaline Phosphatase (AP) Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935168 ICC WB FITC Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935166 ICC WB APC Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN1935169 ICC WB PE Mouse IgG1, kappa AA 456-555 Log in to see
1 ABIN4963890 ELISA FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 AA 265-452 Log in to see 1F3
1 ABIN4963883 ELISA FACS ICC IF IHC IHC (p) FITC Mouse IgG1 AA 265-452 Log in to see 1F3
1 ABIN4963882 ELISA FACS ICC IF IHC IHC (p) WB DyLight 650 Mouse IgG1 AA 265-452 Log in to see 1F3
1 ABIN4963884 ELISA FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 265-452 Log in to see 1F3
1 ABIN4963885 ELISA FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 265-452 Log in to see 1F3
1 ABIN4963887 ELISA FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 AA 265-452 Log in to see 1F3

Top referenced anti-SIRT1 antibody for Immunocytochemistry

Similar anti-SIRT1 Antibodies

Application / Reactivity Monkey Human
ELISA 7 Antibodies 113 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies 30 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 45 Antibodies
Immunofluorescence (IF) 5 Antibodies 62 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 74 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 58 Antibodies
Western Blotting (WB) 9 Antibodies
Chromatin Immunoprecipitation (ChIP) 5 Antibodies
ELISA (Capture) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Reactivity Human, Monkey
(280), (125), (64), (21), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Host Mouse
(186), (79), (27), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(15), (13), (10), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(233), (129), (74), (63), (61), (47), (34), (29), (27), (5), (5), (4), (4), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images
Purification Ascites
Immunogen Purified recombinant fragment of human SIRT1 expressed in E. coli.
Clone 1F3
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molecular Weight 120 kDa
Gene ID 23411
HGNC 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000,
WB: 1/500 - 1/2000,
IHC: 1/200 - 1/1000,
ICC: 1/200 - 1/1000,
FCM: 1/200 - 1/400

Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 Antibody (ABIN969396) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-SIRT1 Antibody (ABIN969396)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, 2009

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 Antibody (ABIN969396) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 1: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 4: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), ...